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Validation Data Gallery

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  • Western Blot (WB) analysis of NR8383 cells using Cxcl2 Recombinant antibody (83758-1-RR)

    WB analysis of NR8383 using 83758-1-RR (same clone as 83758-1-PBS)

    LPS and Brefeldin A treated NR8383 cells were subjected to SDS PAGE followed by western blot with 83758-1-RR (Cxcl2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83758-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using Cxcl2 Recombinant antibody (83758-1-RR)

    IF Staining of THP-1 using 83758-1-RR (same clone as 83758-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed PMA, LPS and Brefeldin A treated THP-1 cells using Cxcl2 antibody (83758-1-RR, Clone: 240367C5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83758-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83758-1-RR

    Affinity and Kinetic Characterization of 83758-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83758-1-RR against Mouse Cxcl2 were performed. The affinity constant is 0.517 nM.

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Rat Cxcl2 Recombinant antibody, PBS Only

Cxcl2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 83758-1-PBS
Clone No.240367C5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

240367C5, CINC-3, C-X-C motif chemokine 2, Cytokine-induced neutrophil chemoattractant 3, Macrophage inflammatory protein 2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83758-1-PBS targets Cxcl2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name chemokine (C-X-C motif) ligand 2
Calculated molecular weight11 kDa
Observed molecular weight10 kDa
GenBank accession numberNM_053647.1
Gene Symbol Cxcl2
Gene ID (NCBI) 114105
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP30348
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CXCL2 is a member of the CXC subfamily, which encodes secreted proteins involved in immunoregulatory and inflammatory processes. CXCL2 is expressed at sites of inflammation and may suppress hematopoietic progenitor cell proliferation. CXCL2 plays an critical role in maintaining cancer-induced macrophage infiltration and the resulting mechanical hypersensitivity and persistent spontaneous nociception (PMID: 36754246).