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  • Featured Product
  • KD/KO Validated

Cyclin A2 Monoclonal antibody, PBS Only

Cyclin A2 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C6C11

Cat no : 66391-1-PBS

Synonyms

CCN1, CCNA, CCNA2, Cyclin A, Cyclin A2, cyclinA2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cyclin A2 Monoclonal antibody (66391-1-Ig)

    WB analysis using 66391-1-Ig (same clone as 66391-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66391-1-Ig (Cyclin A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66391-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using Cyclin A2 Monoclonal antibody (66391-1-Ig)

    WB analysis of HeLa using 66391-1-Ig (same clone as 66391-1-PBS)

    WB result of Cyclin A2 antibody (66391-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cyclin A2 transfected HeLa cells. This data was developed using the same antibody clone with 66391-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Cyclin A2 Monoclonal antibody (66391-1-Ig)

    IHC staining of human tonsillitis using 66391-1-Ig (same clone as 66391-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66391-1-Ig (Cyclin A2 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66391-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Cyclin A2 Monoclonal antibody (66391-1-Ig)

    IHC staining of human tonsillitis using 66391-1-Ig (same clone as 66391-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66391-1-Ig (Cyclin A2 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66391-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Cyclin A2 Monoclonal antibody (66391-1-Ig)

    IF Staining of HeLa using 66391-1-Ig (same clone as 66391-1-PBS)

    Immunofluorescent analysis of (4% PFA ) fixed HeLa cells using 66391-1-Ig(Cyclin A2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66391-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using Cyclin A2 Monoclonal antibody (66391-1-Ig)

    IF Staining of MCF-7 using 66391-1-Ig (same clone as 66391-1-PBS)

    Immunofluorescent analysis of (4% PFA ) fixed MCF-7 cells using 66391-1-Ig(Cyclin A2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66391-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66391-1-PBS targets Cyclin A2 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Cyclin A2 fusion protein Ag12765 相同性解析による交差性が予測される生物種
Full Name cyclin A2
Calculated molecular weight 432 aa, 49 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC104783
Gene symbol CCNA2
Gene ID (NCBI) 890
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CCNA2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. CCNA2 binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions.