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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cyclin D1 Monoclonal antibody (60186-1-Ig)

    WB analysis using 60186-1-Ig (same clone as 60186-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 60186-1-Ig (Cyclin D1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60186-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Cyclin D1 Monoclonal antibody (60186-1-Ig)

    WB analysis using 60186-1-Ig (same clone as 60186-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60186-1-Ig (Cyclin D1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60186-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using Cyclin D1 Monoclonal antibody (60186-1-Ig)

    WB analysis of HeLa using 60186-1-Ig (same clone as 60186-1-PBS)

    WB result of Cyclin D1 antibody (60186-1-Ig, 1:12,000) with si-Control and si-Cyclin D1 transfected HeLa cells. This data was developed using the same antibody clone with 60186-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW 264.7 cells using Cyclin D1 Monoclonal antibody (60186-1-Ig)

    WB analysis of RAW 264.7 using 60186-1-Ig (same clone as 60186-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60186-1-Ig (Cyclin D1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60186-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Cyclin D1 Monoclonal antibody (60186-1-Ig)

    WB analysis using 60186-1-Ig (same clone as 60186-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60186-1-Ig (Cyclin D1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60186-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using Cyclin D1 Monoclonal antibody (60186-1-Ig)

    WB analysis of A549 using 60186-1-Ig (same clone as 60186-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 60186-1-Ig (Cyclin D1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60186-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using Cyclin D1 Monoclonal antibody (60186-1-Ig)

    WB analysis of NIH/3T3 using 60186-1-Ig (same clone as 60186-1-PBS)

    Western blot of Cyclin D1 in HepG2, SW 1990 and NIH/3T3 cell lines with 60186-1-Ig at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60186-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of SH-SY5Y cells using Cyclin D1 Monoclonal antibody (60186-1-Ig)

    FC experiment of SH-SY5Y using 60186-1-Ig (same clone as 60186-1-PBS)

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug Cyclin D1 Monoclonal antibody (60186-1-Ig, Clone:2G3G5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 60186-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

Cyclin D1 Monoclonal antibody, PBS Only

Cyclin D1 Monoclonal Antibody for WB, FC (Intra), ELISA
Cat No. 60186-1-PBS
Clone No.2G3G5

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Synonyms

CCND1, 2G3G5, B cell lymphoma 1 protein, B-cell lymphoma 1 protein, BCL-1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60186-1-PBS targets Cyclin D1 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Cyclin D1 fusion protein Ag0689 相同性解析による交差性が予測される生物種
Full Name cyclin D1
Calculated molecular weight 295 aa, 34 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC000076
Gene Symbol Cyclin D1
Gene ID (NCBI) 595
RRIDAB_10793718
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP24385
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CCND1 (cyclin D1), also known as PRAD1 or BCL1, belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. CCND1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. The CCND1 gene, located on 11q13 has been reported to be overexpressed in mantle cell lymphoma (MCL) due to the chromosomal translocation. CCND1 has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Over-expression of CCND1 is known to correlate with the early onset of cancer and risk of tumor progression and metastasis.