• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

Cyclin D1 Monoclonal antibody, PBS Only

Cyclin D1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2G3G5

Cat no : 60186-1-PBS

Synonyms

B cell lymphoma 1 protein, CCND1, cyclin D, cyclin D1, D11S287E, G1/S specific cyclin D1, PRAD1, PRAD1 oncogene, U21B31

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using Cyclin D1 Monoclonal antibody (60186-1-Ig)

    WB analysis using 60186-1-Ig (same clone as 60186-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60186-1-Ig (Cyclin D1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60186-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Cyclin D1 Monoclonal antibody (60186-1-Ig)

    WB analysis using 60186-1-Ig (same clone as 60186-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60186-1-Ig (Cyclin D1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60186-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using Cyclin D1 Monoclonal antibody (60186-1-Ig)

    WB analysis of HeLa using 60186-1-Ig (same clone as 60186-1-PBS)

    WB result of Cyclin D1 antibody (60186-1-Ig, 1:12,000) with si-Control and si-Cyclin D1 transfected HeLa cells. This data was developed using the same antibody clone with 60186-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW 264.7 cells using Cyclin D1 Monoclonal antibody (60186-1-Ig)

    WB analysis of RAW 264.7 using 60186-1-Ig (same clone as 60186-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60186-1-Ig (Cyclin D1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60186-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using Cyclin D1 Monoclonal antibody (60186-1-Ig)

    WB analysis of NIH/3T3 using 60186-1-Ig (same clone as 60186-1-PBS)

    Western blot of Cyclin D1 in HepG2, SW 1990 and NIH/3T3 cell lines with 60186-1-Ig at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60186-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using Cyclin D1 Monoclonal antibody (60186-1-Ig)

    WB analysis of MCF-7 using 60186-1-Ig (same clone as 60186-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 60186-1-Ig (Cyclin D1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60186-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using Cyclin D1 Monoclonal antibody (60186-1-Ig)

    WB analysis of A549 using 60186-1-Ig (same clone as 60186-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 60186-1-Ig (Cyclin D1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60186-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SH-SY5Y cells using Cyclin D1 Monoclonal antibody (60186-1-Ig)

    WB analysis of SH-SY5Y using 60186-1-Ig (same clone as 60186-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 60186-1-Ig (CCND1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60186-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Cyclin D1 Monoclonal antibody (60186-1-Ig)

    WB analysis using 60186-1-Ig (same clone as 60186-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60186-1-Ig (Cyclin D1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60186-1-PBS in a different storage buffer formulation.

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60186-1-PBS targets Cyclin D1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Cyclin D1 fusion protein Ag0689 相同性解析による交差性が予測される生物種
Full Name cyclin D1
Calculated molecular weight 295 aa, 34 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC000076
Gene symbol Cyclin D1
Gene ID (NCBI) 595
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

CCND1 (cyclin D1), also known as PRAD1 or BCL1, belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. CCND1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. The CCND1 gene, located on 11q13 has been reported to be overexpressed in mantle cell lymphoma (MCL) due to the chromosomal translocation. CCND1 has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Over-expression of CCND1 is known to correlate with the early onset of cancer and risk of tumor progression and metastasis.