Featured Product
KD/KO Validated

Cyclin D3 Polyclonal antibody

Name: Cyclin D3 Polyclonal antibody
Brand: Proteintech
SKU: 26755-1-AP

Cyclin D3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 26755-1-AP

Synonyms

CCND3, cyclin D, Cyclin D3, G1/S specific cyclin D3

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of C6 using 26755-1-AP
C6 cells were subjected to SDS PAGE followed by western blot with 26755-1-AP (Cyclin D3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 26755-1-AP
WB result of Cyclin D3 antibody (26755-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cyclin D3 transfected Jurkat cells.
WB analysis of K-562 using 26755-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 26755-1-AP (Cyclin D3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 26755-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 26755-1-AP (Cyclin D3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 26755-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 26755-1-AP (Cyclin D3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of K-562 using 26755-1-AP
IP result of anti-Cyclin D3 (IP:26755-1-AP, 4ug; Detection:26755-1-AP 1:1000) with K-562 cells lysate 2800ug.
IHC staining of human pancreas using 26755-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 26755-1-AP (Cyclin D3 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human pancreas using 26755-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 26755-1-AP (Cyclin D3 Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of rat kidney using 26755-1-AP
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 26755-1-AP (Cyclin D3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat kidney using 26755-1-AP
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 26755-1-AP (Cyclin D3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas using 26755-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 26755-1-AP (Cyclin D3 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human pancreas using 26755-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 26755-1-AP (Cyclin D3 Antibody) at dilution of 1:200 (under 40x lens).
IF Staining of HeLa using 26755-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 26755-1-AP (Cyclin D3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

-/ -

Cat No. 26755-1-AP

Size/Concentration

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	C6 cells, K-562 cells, Jurkat cells, HeLa cells
Positive IP detected in	K-562 cells
Positive IHC detected in	human pancreas tissue, rat kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 31 publications below
IHC	See 3 publications below
IP	See 1 publications below

Product Information

26755-1-AP targets Cyclin D3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity	human, rat
Cited Reactivity	human, rat, sheep, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Cyclin D3 fusion protein Ag25151 相同性解析による交差性が予測される生物種
Full Name	cyclin D3
Calculated molecular weight	33 kDa
Observed molecular weight	33 kDa
GenBank accession number	BC011616
Gene symbol	Cyclin D3
Gene ID (NCBI)	896
RRID	AB_2756524
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Cyclin D3 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activtiy is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation.

Protocols

Product Specific Protocols
WB protocol for Cyclin D3 antibody 26755-1-AP	Download protocol
IHC protocol for Cyclin D3 antibody 26755-1-AP	Download protocol
IF protocol for Cyclin D3 antibody 26755-1-AP	Download protocol
IP protocol for Cyclin D3 antibody 26755-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IP	Acta Pharmacol Sin Inhibition of USP10 induces myeloma cell apoptosis by promoting cyclin D3 degradation Authors - Yu-Jia Xu KD Validated View Article
human	WB	J Proteome Res Identification of a Proteomic Signature of Senescence in Primary Human Mammary Epithelial Cells Authors - Alireza Delfarah View Article
human	WB	Transl Lung Cancer Res Deubiquitinase USP5 promotes non-small cell lung cancer cell proliferation by stabilizing cyclin D1. Authors - Zhiwei Zhang View Article
human	IHC	Cancer Gene Ther Targeting LRIG2 overcomes resistance to EGFR inhibitor in glioblastoma by modulating GAS6/AXL/SRC signaling. Authors - Minhai Dong View Article
mouse	WB	J Cell Mol Med Cell cycle exit during bortezomib-induced osteogenic differentiation of mesenchymal stem cells was mediated by Xbp1s-upregulated p21Cip1 and p27Kip1. Authors - Dan Zhang View Article
human	WB	Mol Ther Oncolytics S119N Mutation of the E3 Ubiquitin Ligase SPOP Suppresses SLC7A1 Degradation to Regulate Hepatoblastoma Progression. Authors - Weijing He View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cyclin D3 Polyclonal antibody

Cyclin D3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of C6 using 26755-1-AP

WB analysis of Jurkat using 26755-1-AP

WB analysis of K-562 using 26755-1-AP

WB analysis of HeLa using 26755-1-AP

WB analysis of Jurkat using 26755-1-AP

IP experiment of K-562 using 26755-1-AP

IHC staining of human pancreas using 26755-1-AP

IHC staining of human pancreas using 26755-1-AP

IHC staining of rat kidney using 26755-1-AP

IHC staining of rat kidney using 26755-1-AP

IHC staining of human pancreas using 26755-1-AP

IHC staining of human pancreas using 26755-1-AP

IF Staining of HeLa using 26755-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Inhibition of USP10 induces myeloma cell apoptosis by promoting cyclin D3 degradation

Identification of a Proteomic Signature of Senescence in Primary Human Mammary Epithelial Cells

Deubiquitinase USP5 promotes non-small cell lung cancer cell proliferation by stabilizing cyclin D1.

Targeting LRIG2 overcomes resistance to EGFR inhibitor in glioblastoma by modulating GAS6/AXL/SRC signaling.

Cell cycle exit during bortezomib-induced osteogenic differentiation of mesenchymal stem cells was mediated by Xbp1s-upregulated p21Cip1 and p27Kip1.

S119N Mutation of the E3 Ubiquitin Ligase SPOP Suppresses SLC7A1 Degradation to Regulate Hepatoblastoma Progression.