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Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using Cyclin D3 Monoclonal antibody (66357-1-Ig)

    WB analysis of Raji using 66357-1-Ig (same clone as 66357-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 66357-1-Ig (Cyclin D3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66357-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using Cyclin D3 Monoclonal antibody (66357-1-Ig)

    WB analysis of Jurkat using 66357-1-Ig (same clone as 66357-1-PBS)

    WB result of Cyclin D3 antibody (66357-1-Ig; 1:1500; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cyclin D3 transfected Jurkat cells. This data was developed using the same antibody clone with 66357-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using Cyclin D3 Monoclonal antibody (66357-1-Ig)

    WB analysis of HEK-293 using 66357-1-Ig (same clone as 66357-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66357-1-Ig (Cyclin D3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66357-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using Cyclin D3 Monoclonal antibody (66357-1-Ig)

    WB analysis of Jurkat using 66357-1-Ig (same clone as 66357-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66357-1-Ig (Cyclin D3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66357-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using Cyclin D3 Monoclonal antibody (66357-1-Ig)

    WB analysis of K-562 using 66357-1-Ig (same clone as 66357-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 66357-1-Ig (Cyclin D3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66357-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HL-60 cells using Cyclin D3 Monoclonal antibody (66357-1-Ig)

    WB analysis of HL-60 using 66357-1-Ig (same clone as 66357-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 66357-1-Ig (Cyclin D3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66357-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Cyclin D3 Monoclonal antibody (66357-1-Ig)

    IHC staining of human pancreas cancer using 66357-1-Ig (same clone as 66357-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66357-1-Ig (Cyclin D3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66357-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Cyclin D3 Monoclonal antibody (66357-1-Ig)

    IHC staining of human pancreas cancer using 66357-1-Ig (same clone as 66357-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66357-1-Ig (Cyclin D3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66357-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

Cyclin D3 Monoclonal antibody, PBS Only

Cyclin D3 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66357-1-PBS
Clone No.1F2C6

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

CCND3, cyclin D, Cyclin D3, G1/S specific cyclin D3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

66357-1-Ig

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66357-1-PBS targets Cyclin D3 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Cyclin D3 fusion protein Ag22825 相同性解析による交差性が予測される生物種
Full Name cyclin D3
Calculated molecular weight 33 kDa
Observed molecular weight 33-35 kDa
GenBank accession numberBC011616
Gene Symbol Cyclin D3
Gene ID (NCBI) 896
ENSEMBL Gene IDENSG00000112576
RRIDAB_2881736
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP30281
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Cyclin D3 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activitiy is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation.