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Validation Data Gallery

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  • Western Blot (WB) analysis of HT-29 cells using Cyclin E1 Recombinant antibody (82700-10-RR)

    WB analysis of HT-29 using 82700-10-RR (same clone as 82700-10-PBS)

    HT-29 cells were subjected to SDS PAGE followed by western blot with 82700-10-RR (Cyclin E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82700-10-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Cyclin E1 Recombinant antibody (82700-10-RR)

    WB analysis using 82700-10-RR (same clone as 82700-10-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82700-10-RR (Cyclin E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82700-10-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat heart tissue using Cyclin E1 Recombinant antibody (82700-10-RR)

    WB analysis of rat heart using 82700-10-RR (same clone as 82700-10-PBS)

    rat heart tissue were subjected to SDS PAGE followed by western blot with 82700-10-RR (Cyclin E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82700-10-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Cyclin E1 Recombinant antibody (82700-10-RR)

    WB analysis using 82700-10-RR (same clone as 82700-10-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82700-10-RR (Cyclin E antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82700-10-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MCF-7 cells using Cyclin E1 Recombinant antibody (82700-10-RR)

    FC experiment of MCF-7 using 82700-10-RR (same clone as 82700-10-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Cyclin E Recombinant Antibody (82700-10-RR, Clone:240136D4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82700-10-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using Cyclin E1 Recombinant antibody (82700-10-RR)

    FC experiment of HeLa using 82700-10-RR (same clone as 82700-10-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Cyclin E Recombinant Antibody (82700-10-RR, Clone:240136D4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82700-10-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82700-10-RR

    Affinity and Kinetic Characterization of 82700-10-RR

    Biolayer interferometry (BLl) kinetic assays of 82700-10-RR against Human Cyclin E were performed. The affinity constant is 0.564 nM.

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Cyclin E1 Recombinant antibody, PBS Only

Cyclin E1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 82700-10-PBS
Clone No.240136D4

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, FC (Intra), ELISA

Synonyms

CCNE1, G1/S-specific cyclin-E1, G1/S specific cyclin E1, Cyclin E, CCNE

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82700-10-PBS targets Cyclin E1 in WB, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Cyclin E1 fusion protein Ag2110 相同性解析による交差性が予測される生物種
Full Name cyclin E1
Calculated molecular weight 410 aa, 47 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC035498
Gene Symbol Cyclin E1
Gene ID (NCBI) 898
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP24864
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Cyclin E1 (CCNE1) is a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. CCNE1, an essential cyclin activating Cdk2, regulates the G1-S phase transition of the mammalian cell division cycle. Its timing expression plays a direct role in the initiation of DNA replication , the control of histone biosynthesis, and the centrosome cycle. CCNE1 is associated with disease progression in various malignancies and is associated clinically with poor prognosis. Two bands of Cyclin E1 were expressed in U2OS and MDA-MB-231 cells (PMID:9858585, PMID: 24112607).