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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cyclin E1 Recombinant antibody, PBS Only (Detector)

Cyclin E1 Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240136G9

Cat no : 82700-8-PBS

Synonyms

CCNE1, G1/S-specific cyclin-E1, G1/S specific cyclin E1, Cyclin E, CCNE

Validation Data Gallery

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  • Sandwich ELISA standard curve of MP00361-2

    Sandwich ELISA standard curve of MP00361-2

    Sandwich ELISA standard curve of MP00361-2, Human Cyclin Monoclonal Matched Antibody Pair - PBS only. 82700-6-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag2110. 82700-8-PBS was HRP conjugated as the detection antibody. Range: 0.313-40 ng/mL.

  • Sample test of MP00361-2

    Sample test of MP00361-2

    The mean human Cyclin E concentration was determined to be 132.4 ng/mL in Hela cell extract based on a 3.0 mg/mL extract load.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Cyclin E1 Recombinant antibody (82700-8-RR)

    IF Staining of HepG2 using 82700-8-RR (same clone as 82700-8-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Cyclin E antibody (82700-8-RR, Clone: 240136G9 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82700-8-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MCF-7 cells using Cyclin E1 Recombinant antibody (82700-8-RR)

    FC experiment of MCF-7 using 82700-8-RR (same clone as 82700-8-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.2 ug Cyclin E Recombinant antibody (82700-8-RR, Clone:240136G9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.2 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82700-8-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using Cyclin E1 Recombinant antibody (82700-8-RR)

    FC experiment of HeLa using 82700-8-RR (same clone as 82700-8-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Cyclin E Recombinant antibody (82700-8-RR, Clone:240136G9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82700-8-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00361-2

    Cytometric bead array standard curve of MP00361-2

    Cytometric bead array standard curve of MP00361-2, Cyclin E Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82700-6-PBS. Detection antibody: 82700-8-PBS. Standard: Ag2110. Range: 0.313-40 ng/mL.

  • Cytometric bead array standard curve of MP00361-3

    Cytometric bead array standard curve of MP00361-3

    Cytometric bead array standard curve of MP00361-3, Cyclin E Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82700-5-PBS. Detection antibody: 82700-8-PBS. Standard: Ag2110. Range: 0.625-40 ng/mL.

  • Affinity and Kinetic Characterization of 82700-8-RR

    Affinity and Kinetic Characterization of 82700-8-RR

    Biolayer interferometry (BLl) kinetic assays of 82700-8-RR against Human Cyclin E were performed. The affinity constant is 0.229 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82700-8-PBS targets Cyclin E1 as part of a matched antibody pair:

MP00361-2: 82700-6-PBS capture and 82700-8-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP00361-3: 82700-5-PBS capture and 82700-8-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Cyclin E1 fusion protein Ag2110 相同性解析による交差性が予測される生物種
Full Name cyclin E1
Calculated molecular weight 410 aa, 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC035498
Gene symbol Cyclin E1
Gene ID (NCBI) 898
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Cyclin E1 (CCNE1) is a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. CCNE1, an essential cyclin activating Cdk2, regulates the G1-S phase transition of the mammalian cell division cycle. Its timing expression plays a direct role in the initiation of DNA replication , the control of histone biosynthesis, and the centrosome cycle. CCNE1 is associated with disease progression in various malignancies and is associated clinically with poor prognosis. Two bands of Cyclin E1 were expressed in U2OS and MDA-MB-231 cells (PMID:9858585, PMID: 24112607).