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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cyclin E2 Polyclonal antibody, PBS Only

Cyclin E2 Polyclonal Antibody for IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11935-1-PBS

Synonyms

Validation Data Gallery

Filter:
  • Immunoprecipitation (IP) experiment of Jurkat cells using Cyclin E2 Polyclonal antibody (11935-1-AP)

    IP experiment of Jurkat using 11935-1-AP (same clone as 11935-1-PBS)

    IP result of anti-Cyclin E2 (IP:11935-1-AP, 4ug; Detection:11935-1-AP 1:300) with Jurkat cells lysate 4000ug. This data was developed using the same antibody clone with 11935-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Cyclin E2 Polyclonal antibody (11935-1-AP)

    IHC staining of human breast cancer using 11935-1-AP (same clone as 11935-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11935-1-AP (Cyclin E2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11935-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Cyclin E2 Polyclonal antibody (11935-1-AP)

    IHC staining of human breast cancer using 11935-1-AP (same clone as 11935-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11935-1-AP (Cyclin E2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11935-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using Cyclin E2 Polyclonal antibody (11935-1-AP)

    IHC staining of mouse testis using 11935-1-AP (same clone as 11935-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11935-1-AP (Cyclin E2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11935-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using Cyclin E2 Polyclonal antibody (11935-1-AP)

    IHC staining of mouse testis using 11935-1-AP (same clone as 11935-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11935-1-AP (Cyclin E2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11935-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Cyclin E2 Polyclonal antibody (11935-1-AP)

    IF Staining of HeLa using 11935-1-AP (same clone as 11935-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Cyclin E2 antibody (11935-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 11935-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Cyclin E2 Polyclonal antibody (11935-1-AP)

    IF Staining of HeLa using 11935-1-AP (same clone as 11935-1-PBS)

    Immunofluorescent analysis of HeLa cells using 11935-1-AP (Cyclin E2 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 11935-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11935-1-PBS targets Cyclin E2 in IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cyclin E2 fusion protein Ag2532 相同性解析による交差性が予測される生物種
Full Name cyclin E2
Calculated molecular weight 374 aa, 44 kDa
Observed molecular weight 44 kDa
GenBank accession numberBC020729
Gene symbol CCNE2
Gene ID (NCBI) 9134
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Cyclin E2 (CCNE2) belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of cyclindependent kinases (CDKs). Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of cell cycle events. CCNE2 forms a complex with and functions as a regulatory subunit of CDK2 and has been shown to specifically interact with CIP/KIP family of CDK inhibitors. CCNE2 plays a role in cell cycle G1/S transition and its expression peaks at the G1-S phase. Whereas cyclin E1 is expressed in most proliferating normal and tumor cells, cyclin E2 levels are low or undetectable in nontransformed cells, and are elevated in tumor-derived cells.