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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cyclin H Monoclonal antibody, PBS Only

Cyclin H Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

rat, mouse, human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2C5G11

Cat no : 67065-1-PBS

Synonyms

CCNH, 2C5G11, p34, MO15-associated protein, MO15 associated protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cyclin H Monoclonal antibody (67065-1-Ig)

    WB analysis using 67065-1-Ig (same clone as 67065-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67065-1-Ig (Cyclin H antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67065-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Cyclin H Monoclonal antibody (67065-1-Ig)

    WB analysis using 67065-1-Ig (same clone as 67065-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67065-1-Ig (Cyclin H antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67065-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Cyclin H Monoclonal antibody (67065-1-Ig)

    IHC staining of human breast cancer using 67065-1-Ig (same clone as 67065-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67065-1-Ig (Cyclin H antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67065-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Cyclin H Monoclonal antibody (67065-1-Ig)

    IHC staining of human breast cancer using 67065-1-Ig (same clone as 67065-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67065-1-Ig (Cyclin H antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67065-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using Cyclin H Monoclonal antibody (67065-1-Ig)

    IF Staining of PC-3 using 67065-1-Ig (same clone as 67065-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using Cyclin H antibody (67065-1-Ig, Clone: 2C5G11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67065-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of PC-3 cells using Cyclin H Monoclonal antibody (67065-1-Ig)

    FC experiment of PC-3 using 67065-1-Ig (same clone as 67065-1-PBS)

    1X10^6 PC-3 cells were intracellularly stained with 0.4 ug Anti-Human Cyclin H (67065-1-Ig, Clone:2C5G11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67065-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67065-1-PBS targets Cyclin H in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with rat, mouse, human samples.

Tested Reactivity rat, mouse, human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Cyclin H fusion protein Ag28556 相同性解析による交差性が予測される生物種
Full Name cyclin H
Calculated molecular weight 38 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC005280
Gene symbol Cyclin H
Gene ID (NCBI) 902
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.