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Cyclophilin A Polyclonal antibody, PBS Only

Name: Cyclophilin A Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 10720-1-PBS

Cyclophilin A Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Review Product

Cat no : 10720-1-PBS

Synonyms

PPIA, Peptidyl-prolyl cis-trans isomerase A, EC:5.2.1.8, CYPH, CYPA

Validation Data Gallery

Filter:

AllWBIHCIF/ICCFC (Intra)

WB analysis using 10720-1-AP (same clone as 10720-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 10720-1-AP (Cyclophilin A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10720-1-PBS in a different storage buffer formulation.
WB analysis using 10720-1-AP (same clone as 10720-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 10720-1-AP (Cyclophilin A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10720-1-PBS in a different storage buffer formulation.
WB analysis of Raji using 10720-1-AP (same clone as 10720-1-PBS)
Raji cells were subjected to SDS PAGE followed by western blot with 10720-1-AP (Cyclophilin A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10720-1-PBS in a different storage buffer formulation.
WB analysis of C6 using 10720-1-AP (same clone as 10720-1-PBS)
C6 cells were subjected to SDS PAGE followed by western blot with 10720-1-AP (Cyclophilin A antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10720-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 10720-1-AP (same clone as 10720-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10720-1-AP (Cyclophilin A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10720-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 10720-1-AP (same clone as 10720-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10720-1-AP (Cyclophilin A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10720-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 10720-1-AP (same clone as 10720-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer using 10720-1-AP (Cyclophilin A antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10720-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 10720-1-AP (same clone as 10720-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer using 10720-1-AP (Cyclophilin A antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 10720-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 10720-1-AP (same clone as 10720-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 10720-1-AP (Cyclophilin A antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 10720-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 10720-1-AP (same clone as 10720-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta using 10720-1-AP (Cyclophilin A antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10720-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 10720-1-AP (same clone as 10720-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta using 10720-1-AP (Cyclophilin A antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 10720-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 10720-1-AP (same clone as 10720-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 10720-1-AP (Cyclophilin A antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10720-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 10720-1-AP (same clone as 10720-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis using 10720-1-AP (Cyclophilin A antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10720-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 10720-1-AP (same clone as 10720-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis using 10720-1-AP (Cyclophilin A antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 10720-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 10720-1-AP (same clone as 10720-1-PBS)
Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Cyclophilin A antibody (10720-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10720-1-PBS in a different storage buffer formulation.
FC experiment of HeLa using 10720-1-AP (same clone as 10720-1-PBS)
1x10^6 HeLa cells were intracellularly stained with 0.25 ug Cyclophilin A Polyclonal antibody (10720-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 10720-1-PBS in a different storage buffer formulation.
×
1x10^6 HeLa cells were intracellularly stained with 0.25 ug Cyclophilin A Polyclonal antibody (10720-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 10720-1-PBS in a different storage buffer formulation.

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Cat No. 10720-1-PBS

Size/Concentration

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Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10720-1-PBS targets Cyclophilin A in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Cyclophilin A fusion protein Ag1120 相同性解析による交差性が予測される生物種
Full Name	peptidylprolyl isomerase A (cyclophilin A)
Calculated molecular weight	18 kDa
Observed molecular weight	18 kDa
GenBank accession number	BC005320
Gene symbol	PPIA
Gene ID (NCBI)	5478
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

Cyclophilin A, also named as PPIA and Rotamase A, belongs to the cyclophilin-type PPIase family and PPIase A subfamily. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIA forms a trimolecular complex with cyclophilin and calcineurins to inhibit calcineurin phosphatase activity. PPIA is incorporated into the virion of the type 1 human immunodeficiency virus (HIV-1) via a direct interaction with the capsid domain of the viral Gag polyprotein and is crucial for efficient viral replication.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cyclophilin A Polyclonal antibody, PBS Only

Cyclophilin A Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Validation Data Gallery

WB analysis using 10720-1-AP (same clone as 10720-1-PBS)

WB analysis using 10720-1-AP (same clone as 10720-1-PBS)

WB analysis of Raji using 10720-1-AP (same clone as 10720-1-PBS)

WB analysis of C6 using 10720-1-AP (same clone as 10720-1-PBS)

IHC staining of human colon cancer using 10720-1-AP (same clone as 10720-1-PBS)

IHC staining of human colon cancer using 10720-1-AP (same clone as 10720-1-PBS)

IHC staining of human colon cancer using 10720-1-AP (same clone as 10720-1-PBS)

IHC staining of human colon cancer using 10720-1-AP (same clone as 10720-1-PBS)

IHC staining of human testis using 10720-1-AP (same clone as 10720-1-PBS)

IHC staining of human placenta using 10720-1-AP (same clone as 10720-1-PBS)

IHC staining of human placenta using 10720-1-AP (same clone as 10720-1-PBS)

IHC staining of human testis using 10720-1-AP (same clone as 10720-1-PBS)

IHC staining of human tonsillitis using 10720-1-AP (same clone as 10720-1-PBS)

IHC staining of human tonsillitis using 10720-1-AP (same clone as 10720-1-PBS)

IF Staining of HepG2 using 10720-1-AP (same clone as 10720-1-PBS)

FC experiment of HeLa using 10720-1-AP (same clone as 10720-1-PBS)

Tested Applications

Recommended dilution

Product Information

Background Information