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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cyclophilin A Monoclonal antibody (67880-1-Ig)

    WB analysis using 67880-1-Ig (same clone as 67880-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67880-1-Ig (CYPA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67880-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Cyclophilin A Monoclonal antibody (67880-1-Ig)

    WB analysis using 67880-1-Ig (same clone as 67880-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67880-1-Ig (CYPA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67880-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Cyclophilin A Monoclonal antibody (67880-1-Ig)

    IF Staining of HeLa using 67880-1-Ig (same clone as 67880-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cyclophilin A antibody (67880-1-Ig, Clone: 2B11B9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67880-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using Cyclophilin A Monoclonal antibody (67880-1-Ig)

    FC experiment of HeLa using 67880-1-Ig (same clone as 67880-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Cyclophilin A (67880-1-Ig, Clone:2B11B9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67880-1-PBS in a different storage buffer formulation.

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Cyclophilin A Monoclonal antibody, PBS Only

Cyclophilin A Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 67880-1-PBS
Clone No.2B11B9

Host / Isotype

Mouse / IgG1

Reactivity

rat, mouse, human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

PPIA, EC:5.2.1.8, CYPH, CYPA, Cyclosporin A-binding protein

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration:  100 μg, 1mg/ml
100 μg, 1mg/ml

¥167,000/ 100 μg

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67880-1-PBS targets Cyclophilin A in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with rat, mouse, human samples.

Tested Reactivity rat, mouse, human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Cyclophilin A fusion protein Ag1120 相同性解析による交差性が予測される生物種
Full Name peptidylprolyl isomerase A (cyclophilin A)
Calculated molecular weight 18 kDa
Observed molecular weight18 kDa
GenBank accession numberBC005320
Gene Symbol PPIA
Gene ID (NCBI) 5478
RRIDAB_2918637
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP62937
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Cyclophilin A, also named as PPIA and Rotamase A, belongs to the cyclophilin-type PPIase family and PPIase A subfamily. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIA forms a trimolecular complex with cyclophilin and calcineurins to inhibit calcineurin phosphatase activity. PPIA is incorporated into the virion of the type 1 human immunodeficiency virus (HIV-1) via a direct interaction with the capsid domain of the viral Gag polyprotein and is crucial for efficient viral replication.