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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cyp4a12a Polyclonal antibody

Cyp4a12a Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat, human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 51157-1-AP

Synonyms

Cyp4a12, Cyp4a12a

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse liver tissue using Cyp4a12a Polyclonal antibody (51157-1-AP)

    WB analysis of mouse liver using 51157-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 51157-1-AP (Cyp4a12a antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat liver tissue using Cyp4a12a Polyclonal antibody (51157-1-AP)

    IHC staining of rat liver using 51157-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 51157-1-AP (Cyp4a12a antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat liver tissue using Cyp4a12a Polyclonal antibody (51157-1-AP)

    IHC staining of rat liver using 51157-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 51157-1-AP (Cyp4a12a antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse liver tissue
Positive IHC detected inrat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 51157-1-AP is Cyp4a12a Fusion Protein expressed in E. coli.

Tested Reactivity mouse, rat, human
Cited Reactivity rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cyp4a12a fusion protein Ag0509 相同性解析による交差性が予測される生物種
Full Name cytochrome P450, family 4, subfamily a, polypeptide 12a
Observed molecular weight 58-70 kDa
GenBank accession numberNM_177406
Gene symbol Cyp4a12a
Gene ID (NCBI) 277753
RRIDAB_2881255
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. Mouse Cyp4a12a oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. For modification, the MW of Cyp4a12a is about 58-70 kDa in western blot detection.

Protocols

Product Specific Protocols
WB protocol for Cyp4a12a antibody 51157-1-APDownload protocol
IHC protocol for Cyp4a12a antibody 51157-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Exp Cell Res

Effects of PPARs/20-HETE on the renal impairment under diabetic conditions.

Authors - Shumei Ding
View Article