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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cystatin C Recombinant antibody (83422-1-RR)

    WB analysis using 83422-1-RR (same clone as 83422-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83422-1-RR (Cystatin C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83422-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using Cystatin C Recombinant antibody (83422-1-RR)

    IHC staining of mouse kidney using 83422-1-RR (same clone as 83422-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 83422-1-RR (cystatin c antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83422-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using Cystatin C Recombinant antibody (83422-1-RR)

    IHC staining of mouse kidney using 83422-1-RR (same clone as 83422-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 83422-1-RR (cystatin c antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83422-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83422-1-RR

    Affinity and Kinetic Characterization of 83422-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83422-1-RR against Mouse Cystatin C were performed. The affinity constant is 20.3 nM.

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Mouse Cystatin C Recombinant antibody, PBS Only

Cystatin C Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 83422-1-PBS
Clone No.240202A2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

Cst3, CysC, 240202A2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83422-1-PBS targets Cystatin C in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name cystatin C
Calculated molecular weight15KD
Observed molecular weight 13-16 kDa
GenBank accession numberNM_009976.4
Gene Symbol Cystatin C
Gene ID (NCBI) 13010
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP21460
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Cystatin C is a 13-kDa inhibitor of cysteine proteinases which is secreted by all cell types and is completely cleared from the organism through glomerular filtration, shown to be an early and sensitive biomarker of renal dysfunction. It is also used as an emerging biomarker in cardiovascular disease. Cystatin C is involved in a variety of inflammatory reactions. The concentration of serum cystatin C has also been shown to be unaltered in certain inflammatory conditions or other disorders of metabolism. The plasma level of serum cystatin C can be expressed as its level of generation from cells and diet and its subsequent elimination through the gut, liver, and kidney.