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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cytochrome P450 Reductase Polyclonal antibody (29814-1-AP)

    WB analysis using 29814-1-AP

    Human placenta tissue were subjected to SDS PAGE followed by western blot with 29814-1-AP (Cytochrome P450 Reductase antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Cytochrome P450 Reductase Polyclonal antibody (29814-1-AP)

    WB analysis using 29814-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29814-1-AP (Cytochrome P450 Reductase antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using Cytochrome P450 Reductase Polyclonal antibody (29814-1-AP)

    WB analysis of HepG2 using 29814-1-AP

    WB result of Cytochrome P450 Reductase antibody (29814-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cytochrome P450 Reductase transfected HepG2 cells.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using Cytochrome P450 Reductase Polyclonal antibody (29814-1-AP)

    IHC staining of mouse kidney using 29814-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 29814-1-AP (Cytochrome P450 Reductase antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat kidney tissue using Cytochrome P450 Reductase Polyclonal antibody (29814-1-AP)

    IHC staining of rat kidney using 29814-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 29814-1-AP (Cytochrome P450 Reductase antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cytochrome P450 Reductase Polyclonal antibody

Cytochrome P450 Reductase Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29814-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

POR, P450R, CYPOR, CPR

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman placenta tissue, HepG2 cells, mouse liver tissue, rat liver tissue
Positive IHC detected inmouse kidney tissue, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:1500-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

29814-1-AP targets Cytochrome P450 Reductase in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cytochrome P450 Reductase fusion protein Ag30899 相同性解析による交差性が予測される生物種
Full Name P450 (cytochrome) oxidoreductase
Calculated molecular weight 77 kDa
Observed molecular weight77 kDa
GenBank accession numberNM_000941
Gene Symbol Cytochrome P450 Reductase
Gene ID (NCBI) 5447
RRIDAB_3086171
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP16435
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cytochrome P450 Reductase (CYPOR) consists of the flavin mononucleotide (FMN) domain and the flavin adenine dinucleotide (FAD) domain, which bind the corresponding cofactors and transfer electrons from NADPH via FAD and FMN (PMID: 33498551). CYPOR is an essential component of the microsomal P450 mixed-function oxidase system and is required for electron transfer from NADPH to cytochromes P450 in microsomes. The calculated molecular weight of CYPOR is 77 kDa.

Protocols

Product Specific Protocols
WB protocol for Cytochrome P450 Reductase antibody 29814-1-APDownload protocol
IHC protocol for Cytochrome P450 Reductase antibody 29814-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

iScience

AMPD3 promotes doxorubicin-induced cardiomyopathy through HSP90α-mediated ferroptosis

Authors - Liting Cheng
View Article