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  • Featured Product
  • KD/KO Validated

Cytohesin 2 Monoclonal antibody, PBS Only

Cytohesin 2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3A7A1

Cat no : 67185-1-PBS

Synonyms

ARF exchange factor, ARNO, CTS18, CTS18.1, CYTH2, cytohesin 2, Protein ARNO, PSCD2, PSCD2L, SEC7L, Sec7p L, Sec7p like

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cytohesin 2 Monoclonal antibody (67185-1-Ig)

    WB analysis using 67185-1-Ig (same clone as 67185-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67185-1-Ig (CYTH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67185-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Cytohesin 2 Monoclonal antibody (67185-1-Ig)

    IHC staining of human liver cancer using 67185-1-Ig (same clone as 67185-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67185-1-Ig (Cytohesin 2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67185-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Cytohesin 2 Monoclonal antibody (67185-1-Ig)

    IHC staining of human liver cancer using 67185-1-Ig (same clone as 67185-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67185-1-Ig (Cytohesin 2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67185-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Cytohesin 2 Monoclonal antibody (67185-1-Ig)

    IHC staining of human colon cancer using 67185-1-Ig (same clone as 67185-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67185-1-Ig (Cytohesin 2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67185-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Cytohesin 2 Monoclonal antibody (67185-1-Ig)

    IHC staining of human colon cancer using 67185-1-Ig (same clone as 67185-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67185-1-Ig (Cytohesin 2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67185-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Cytohesin 2 Monoclonal antibody (67185-1-Ig)

    IF Staining of HeLa using 67185-1-Ig (same clone as 67185-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 67185-1-Ig (Cytohesin 2 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67185-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67185-1-PBS targets Cytohesin 2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Cytohesin 2 fusion protein Ag21279 相同性解析による交差性が予測される生物種
Full Name cytohesin 2
Calculated molecular weight 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC004361
Gene symbol Cytohesin 2
Gene ID (NCBI) 9266
RRIDAB_2882481
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Pleckstrin homology, Sec7 and coiled/coil domains 2 (PSCD2) is a member of the PSCD family. Members of this family have identical structural organization that consists of an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein (GEP) activity, and the PH domain interacts with phospholipids and is responsible for association of PSCDs with membranes. Members of this family appear to mediate the regulation of protein sorting and membrane trafficking. PSCD2 exhibits GEP activity in vitro with small G proteins ARF1, ARF3, and ARF6.