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  • Featured Product
  • KD/KO Validated

Cytokeratin 13 Monoclonal antibody, PBS Only (Capture)

Cytokeratin 13 Monoclonal Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E10G2

Cat no : 66684-1-PBS

Synonyms

KRT13, Cytokeratin,Keratin, ck17, CK-13, CK13

Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using Cytokeratin 13 Monoclonal antibody (66684-1-Ig)

    WB analysis of A431 using 66684-1-Ig (same clone as 66684-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 66684-1-Ig (KRT13 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66684-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using Cytokeratin 13 Monoclonal antibody (66684-1-Ig)

    WB analysis of A431 using 66684-1-Ig (same clone as 66684-1-PBS)

    WB result of Cytokeratin 13 antibody (66684-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cytokeratin 13 transfected A431 cells. This data was developed using the same antibody clone with 66684-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat skin tissue using Cytokeratin 13 Monoclonal antibody (66684-1-Ig)

    WB analysis of rat skin using 66684-1-Ig (same clone as 66684-1-PBS)

    rat skin tissue were subjected to SDS PAGE followed by western blot with 66684-1-Ig (KRT13 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66684-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using Cytokeratin 13 Monoclonal antibody (66684-1-Ig)

    IHC staining of human cervical cancer using 66684-1-Ig (same clone as 66684-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66684-1-Ig (KRT13 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66684-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using Cytokeratin 13 Monoclonal antibody (66684-1-Ig)

    IHC staining of human cervical cancer using 66684-1-Ig (same clone as 66684-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66684-1-Ig (KRT13 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66684-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using Cytokeratin 13 Monoclonal antibody (66684-1-Ig)

    IHC staining of human oesophagus cancer using 66684-1-Ig (same clone as 66684-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 66684-1-Ig (Cytokeratin 13 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66684-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using Cytokeratin 13 Monoclonal antibody (66684-1-Ig)

    IHC staining of human oesophagus cancer using 66684-1-Ig (same clone as 66684-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 66684-1-Ig (Cytokeratin 13 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66684-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using Cytokeratin 13 Monoclonal antibody (66684-1-Ig)

    IHC staining of human cervical cancer using 66684-1-Ig (same clone as 66684-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66684-1-Ig (Cytokeratin 13 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66684-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using Cytokeratin 13 Monoclonal antibody (66684-1-Ig)

    IHC staining of human oesophagus cancer using 66684-1-Ig (same clone as 66684-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 66684-1-Ig (Cytokeratin 13 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66684-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using Cytokeratin 13 Monoclonal antibody (66684-1-Ig)

    IHC staining of human oesophagus cancer using 66684-1-Ig (same clone as 66684-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 66684-1-Ig (Cytokeratin 13 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66684-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human cervical cancer tissue using Cytokeratin 13 Monoclonal antibody (66684-1-Ig)

    IF Staining of human cervical cancer using 66684-1-Ig (same clone as 66684-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human cervical cancer tissue using Cytokeratin 13 antibody (66684-1-Ig, Clone: 1E10G2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66684-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51141-1

    Cytometric bead array standard curve of MP51141-1

    Cytometric bead array standard curve of MP51141-1, Cytokeratin 13 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66684-1-PBS. Detection antibody: 66684-2-PBS. Standard:Ag0217. Range: 0.098-100 ng/mL.

  • Cytometric bead array standard curve of MP51141-3

    Cytometric bead array standard curve of MP51141-3

    Cytometric bead array standard curve of MP51141-3, Cytokeratin 13 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66684-1-PBS. Detection antibody: 66684-4-PBS. Standard:Ag0217. Range: 0.098-100 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66684-1-PBS targets Cytokeratin 13 as part of a matched antibody pair:

MP51141-1: 66684-1-PBS capture and 66684-2-PBS detection (validated in Cytometric bead array)

MP51141-3: 66684-1-PBS capture and 66684-4-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Cytokeratin 13 fusion protein Ag0217 相同性解析による交差性が予測される生物種
Full Name keratin 13
Calculated molecular weight 50 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC002661
Gene symbol Cytokeratin 13
Gene ID (NCBI) 3860
RRIDAB_2882038
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. The Keratin 13 (KRT13) gene encodes a type I acidic keratin which is expressed in the differentiated cells of non-cornified stratified squamous epithelia. This antibody can react with Cytokeratin 15.