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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cytokeratin 13 Recombinant antibody, PBS Only

Cytokeratin 13 Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230366E8

Cat no : 83058-4-PBS

Synonyms

KRT13, Cytokeratin,Keratin, ck17, CK-13, CK13

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A431 cells using Cytokeratin 13 Recombinant antibody (83058-4-RR)

    IF Staining of A431 using 83058-4-RR (same clone as 83058-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using Cytokeratin 13 antibody (83058-4-RR, Clone: 230366E8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83058-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A431 cells using Cytokeratin 13 Recombinant antibody (83058-4-RR)

    FC experiment of A431 using 83058-4-RR (same clone as 83058-4-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Anti-Human KRT13 (83058-4-RR, Clone:230366E8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83058-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83058-4-RR

    Affinity and Kinetic Characterization of 83058-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83058-4-RR against Human Cytokeratin 13 were performed. The affinity constant is 1.65 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83058-4-PBS targets Cytokeratin 13 in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Cytokeratin 13 fusion protein Ag0217 相同性解析による交差性が予測される生物種
Full Name keratin 13
Calculated molecular weight 50 kDa
GenBank accession numberBC002661
Gene symbol Cytokeratin 13
Gene ID (NCBI) 3860
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Keratin 13 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. Mutations in keratin 13 gene and keratin 4 have been associated with the autosomal dominant disorder White Sponge Nevus. The type I cytokeratins are clustered in a region of chromosome 17q21.2.