Cytokeratin 14 Recombinant antibody
Cytokeratin 14 Recombinant Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
1H22
Cat no : 83379-1-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | rat skin tissue |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:2800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
The immunogen of 83379-1-RR is Cytokeratin 14 Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | Cytokeratin 14 fusion protein Ag0188 相同性解析による交差性が予測される生物種 |
Full Name | keratin 14 |
Calculated molecular weight | 472 aa, 52 kDa |
Observed molecular weight | 52 kDa |
GenBank accession number | BC002690 |
Gene symbol | Cytokeratin 14 |
Gene ID (NCBI) | 3861 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Cytokeratin 14, one of about 20 different cytokeratin isotypes of human cells, is the intermediate filament protein characteristic of epithelial cells. Cytokeratin 14 is expressed in the basal compartment of all stratified squamous epithelia. In various kinds of human tumors, the appearance and increasing expression of Cytokeratin 14 were strikingly associated with higher grade and stage of carcinoma, with varying degrees of unfavorable prognosis. In lung squamous cell carcinoma(LSCC), Cytokeratin 14 was expressed in the tumor cell nests showing stromal invasion with fibrosis and lymph node metastases, indicating that Cytokeratin 14 involved in proliferation and metastasis of LSCC. Cytokeratin 14 expression is sometimes used in diagnosis of myoepithelioma1 and intraductal vs. invasive ductal carcinoma of the breast.
Protocols
Product Specific Protocols | |
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WB protocol for Cytokeratin 14 antibody 83379-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |