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Validation Data Gallery

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  • Western Blot (WB) analysis of rat skin tissue using Cytokeratin 14 Recombinant antibody (83379-1-RR)

    WB analysis of rat skin using 83379-1-RR

    rat skin tissue were subjected to SDS PAGE followed by western blot with 83379-1-RR (KRT14 antibody) at dilution of 1:1400 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83379-1-RR

    Affinity and Kinetic Characterization of 83379-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83379-1-RR against Human Cytokeratin 14 were performed. The affinity constant is 43.1 nM.

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Cytokeratin 14 Recombinant antibody

Cytokeratin 14 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 83379-1-RR
Clone No.1H22

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

KRT14, Cytokeratin-14, Cytokeratin,Keratin, CK-14, CK14

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inrat skin tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83379-1-RR targets Cytokeratin 14 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Cytokeratin 14 fusion protein Ag0188 相同性解析による交差性が予測される生物種
Full Name keratin 14
Calculated molecular weight 472 aa, 52 kDa
Observed molecular weight52 kDa
GenBank accession numberBC002690
Gene Symbol Cytokeratin 14
Gene ID (NCBI) 3861
RRIDAB_3671033
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP02533
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cytokeratin 14, one of about 20 different cytokeratin isotypes of human cells, is the intermediate filament protein characteristic of epithelial cells. Cytokeratin 14 is expressed in the basal compartment of all stratified squamous epithelia. In various kinds of human tumors, the appearance and increasing expression of Cytokeratin 14 were strikingly associated with higher grade and stage of carcinoma, with varying degrees of unfavorable prognosis. In lung squamous cell carcinoma(LSCC), Cytokeratin 14 was expressed in the tumor cell nests showing stromal invasion with fibrosis and lymph node metastases, indicating that Cytokeratin 14 involved in proliferation and metastasis of LSCC. Cytokeratin 14 expression is sometimes used in diagnosis of myoepithelioma1 and intraductal vs. invasive ductal carcinoma of the breast.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 14 antibody 83379-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols