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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cytokeratin 15 Monoclonal antibody, PBS Only (Capture)

Cytokeratin 15 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

6B4F8

Cat no : 60247-1-PBS

Synonyms

CK 15, CK15, Cytokeratin 15, K15, K1CO, keratin 15, KRT15, KRTB

Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using Cytokeratin 15 Monoclonal antibody (60247-1-Ig)

    WB analysis of A431 using 60247-1-Ig (same clone as 60247-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 60247-1-Ig (Cytokeratin 15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60247-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP50002-1

    Sandwich ELISA standard curve of MP50002-1

    Sandwich ELISA standard curve ofMP50002-1, KRT15 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60247-1-PBS. Detection antibody: HRP-conjugated 60247-2-PBS. Standard: Ag0185. Range: 0.313-20 ng/mL.

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using Cytokeratin 15 Monoclonal antibody (60247-1-Ig)

    IHC staining of human oesophagus cancer using 60247-1-Ig (same clone as 60247-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60247-1-Ig (Cytokeratin 15 antibody at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 60247-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using Cytokeratin 15 Monoclonal antibody (60247-1-Ig)

    IHC staining of human oesophagus cancer using 60247-1-Ig (same clone as 60247-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60247-1-Ig (Cytokeratin 15 antibody at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 60247-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using Cytokeratin 15 Monoclonal antibody (60247-1-Ig)

    IHC staining of human skin cancer using 60247-1-Ig (same clone as 60247-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 60247-1-Ig (Cytokeratin 15 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60247-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using Cytokeratin 15 Monoclonal antibody (60247-1-Ig)

    IHC staining of human skin cancer using 60247-1-Ig (same clone as 60247-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 60247-1-Ig (Cytokeratin 15 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60247-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Cytokeratin 15 Monoclonal antibody (60247-1-Ig)

    IF Staining of HeLa using 60247-1-Ig (same clone as 60247-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Cytokeratin 15 antibody (60247-1-Ig, Clone: 6B4F8 ) at dilution of 1:25 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60247-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50002-1

    Cytometric bead array standard curve of MP50002-1

    Cytometric bead array standard curve ofMP50002-1, KRT15 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60247-1-PBS. Detection antibody: 60247-2-PBS. Standard: Ag0185. Range: 1.563-100 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60247-1-PBS targets Cytokeratin 15 as part of a matched antibody pair:

MP50002-1: 60247-1-PBS capture and 60247-2-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Cytokeratin 15 fusion protein Ag0185 相同性解析による交差性が予測される生物種
Full Name keratin 15
Calculated molecular weight 49 kDa
Observed molecular weight 49 kDa
GenBank accession numberBC002641
Gene symbol KRT15
Gene ID (NCBI) 3866
RRIDAB_2881369
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer 100% PBS pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18). Keratin 15 is a type I cytokeratin. It is found in some progenitor basal cells within complex epithelia.