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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cytokeratin 17-specific Recombinant antibody (84978-1-RR)

    WB analysis using 84978-1-RR (same clone as 84978-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84978-1-RR (KRT17-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84978-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using Cytokeratin 17-specific Recombinant antibody (84978-1-RR)

    IHC staining of human skin cancer using 84978-1-RR (same clone as 84978-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 84978-1-RR (Cytokeratin 17-specific antibody) at dilution of 1:6000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84978-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using Cytokeratin 17-specific Recombinant antibody (84978-1-RR)

    IHC staining of human skin cancer using 84978-1-RR (same clone as 84978-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 84978-1-RR (Cytokeratin 17-specific antibody) at dilution of 1:6000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84978-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using Cytokeratin 17-specific Recombinant antibody (84978-1-RR)

    IHC staining of human skin cancer using 84978-1-RR (same clone as 84978-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 84978-1-RR (Cytokeratin 17-specific antibody) at dilution of 1:6000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84978-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using Cytokeratin 17-specific Recombinant antibody (84978-1-RR)

    IHC staining of human cervical cancer using 84978-1-RR (same clone as 84978-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 84978-1-RR (Cytokeratin 17-specific antibody) at dilution of 1:6000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84978-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using Cytokeratin 17-specific Recombinant antibody (84978-1-RR)

    IHC staining of human cervical cancer using 84978-1-RR (same clone as 84978-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 84978-1-RR (Cytokeratin 17-specific antibody) at dilution of 1:6000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84978-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using Cytokeratin 17-specific Recombinant antibody (84978-1-RR)

    IHC staining of human cervical cancer using 84978-1-RR (same clone as 84978-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 84978-1-RR (Cytokeratin 17-specific antibody) at dilution of 1:6000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84978-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Cytokeratin 17-specific Recombinant antibody (84978-1-RR)

    IF Staining of HeLa using 84978-1-RR (same clone as 84978-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cytokeratin 17-Specific antibody (84978-1-RR, Clone: 242240A8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84978-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using Cytokeratin 17-specific Recombinant antibody (84978-1-RR)

    IF Staining of A431 using 84978-1-RR (same clone as 84978-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Cytokeratin 17-Specific antibody (84978-1-RR, Clone: 242240A8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84978-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using Cytokeratin 17-specific Recombinant antibody (84978-1-RR)

    FC experiment of HeLa using 84978-1-RR (same clone as 84978-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Cytokeratin 17-specific Recombinant antibody (84978-1-RR, Clone:242240A8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84978-1-PBS in a different storage buffer formulation.

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Cytokeratin 17-specific Recombinant antibody, PBS Only

Cytokeratin 17-specific Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84978-1-PBS
Clone No.242240A8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

Cytokeratin 17, KRT17, 242240A8, CK 17, CK-17

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84978-1-PBS targets Cytokeratin 17-specific in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name keratin 17
Calculated molecular weight 48 kDa
Observed molecular weight48 kDa
GenBank accession numberNM_000422
Gene Symbol Cytokeratin 17
Gene ID (NCBI) 3872
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ04695
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Keratins are a large family of proteins that form the intermediate filaments that make up the cytoskeleton. Type I keratins are a group of acidic intermediate filament proteins and type II keratins are the basic or neutral counterparts. Keratin 17 is encoded by the KRT17 gene and is a type I cytokeratin found in nail beds, hair follicles, sebaceous glands, and epidermal appendages.The molecular weight of cytokeratin 17 is approximately 49 kDa.Cytokeratin 17 plays a role in the formation and maintenance of epidermal appendages, especially in determining the shape and orientation of hair. It is required for the maintenance of the anagen (growth) state of hair follicles by modulating the function of TNF-alpha for hair cycling and is also involved in tissue repair (PMID: 16702408).