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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cytokeratin 17-specific Recombinant antibody, PBS Only

Cytokeratin 17-specific Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

242240A8

Cat no : 84978-1-PBS

Synonyms

Cytokeratin 17, KRT17, Cytokeratin-17, Cytokeratin,Keratin, CK-17

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cytokeratin 17-specific Recombinant antibody (84978-1-RR)

    WB analysis using 84978-1-RR (same clone as 84978-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84978-1-RR (KRT17-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84978-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using Cytokeratin 17-specific Recombinant antibody (84978-1-RR)

    IHC staining of human skin cancer using 84978-1-RR (same clone as 84978-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 84978-1-RR (Cytokeratin 17-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84978-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using Cytokeratin 17-specific Recombinant antibody (84978-1-RR)

    IHC staining of human skin cancer using 84978-1-RR (same clone as 84978-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 84978-1-RR (Cytokeratin 17-Specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84978-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Cytokeratin 17-specific Recombinant antibody (84978-1-RR)

    IHC staining of human colon cancer using 84978-1-RR (same clone as 84978-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84978-1-RR (Cytokeratin 17-Specific antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84978-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Cytokeratin 17-specific Recombinant antibody (84978-1-RR)

    IHC staining of human colon cancer using 84978-1-RR (same clone as 84978-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84978-1-RR (Cytokeratin 17-Specific antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84978-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Cytokeratin 17-specific Recombinant antibody (84978-1-RR)

    IF Staining of HeLa using 84978-1-RR (same clone as 84978-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cytokeratin 17-Specific antibody (84978-1-RR, Clone: 242240A8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84978-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using Cytokeratin 17-specific Recombinant antibody (84978-1-RR)

    IF Staining of A431 using 84978-1-RR (same clone as 84978-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Cytokeratin 17-Specific antibody (84978-1-RR, Clone: 242240A8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84978-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84978-1-PBS targets Cytokeratin 17-specific in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name keratin 17
Calculated molecular weight 48 kDa
Observed molecular weight48 kDa
GenBank accession numberNM_000422
Gene symbol Cytokeratin 17
Gene ID (NCBI) 3872
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Keratins are a large family of proteins that form the intermediate filaments that make up the cytoskeleton. Type I keratins are a group of acidic intermediate filament proteins and type II keratins are the basic or neutral counterparts. Keratin 17 is encoded by the KRT17 gene and is a type I cytokeratin found in nail beds, hair follicles, sebaceous glands, and epidermal appendages.The molecular weight of cytokeratin 17 is approximately 49 kDa.Cytokeratin 17 plays a role in the formation and maintenance of epidermal appendages, especially in determining the shape and orientation of hair. It is required for the maintenance of the anagen (growth) state of hair follicles by modulating the function of TNF-alpha for hair cycling and is also involved in tissue repair (PMID: 16702408).