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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cytokeratin 19 Polyclonal antibody

Cytokeratin 19 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 29855-1-AP

Synonyms

CK19, CK 19, KRT19, K19, Cytokeratin-19

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cytokeratin 19 Polyclonal antibody (29855-1-AP)

    WB analysis using 29855-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 29855-1-AP (Cytokeratin 19 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of BxPC-3 cells using Cytokeratin 19 Polyclonal antibody (29855-1-AP)

    WB analysis of BxPC-3 using 29855-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 29855-1-AP (Cytokeratin 19 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Cytokeratin 19 Polyclonal antibody (29855-1-AP)

    IHC staining of human breast cancer using 29855-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 29855-1-AP (Cytokeratin 19 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Cytokeratin 19 Polyclonal antibody (29855-1-AP)

    IHC staining of human breast cancer using 29855-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 29855-1-AP (Cytokeratin 19 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using Cytokeratin 19 Polyclonal antibody (29855-1-AP)

    IHC staining of human colon using 29855-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29855-1-AP (Cytokeratin 19 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using Cytokeratin 19 Polyclonal antibody (29855-1-AP)

    IHC staining of human colon using 29855-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29855-1-AP (Cytokeratin 19 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using Cytokeratin 19 Polyclonal antibody (29855-1-AP)

    IHC staining of human thyroid cancer using 29855-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 29855-1-AP (Cytokeratin 19 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using Cytokeratin 19 Polyclonal antibody (29855-1-AP)

    IHC staining of human thyroid cancer using 29855-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 29855-1-AP (Cytokeratin 19 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human normal colon using Cytokeratin 19 Polyclonal antibody (29855-1-AP)

    IHC staining of human normal colon using 29855-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 29855-1-AP (Cytokeratin 19 antibody) at dilution of 1:8000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Cytokeratin 19 Polyclonal antibody (29855-1-AP)

    IF Staining of HeLa using 29855-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cytokeratin 19 antibody (29855-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using Cytokeratin 19 Polyclonal antibody (29855-1-AP)

    IF Staining of A549 using 29855-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using Cytokeratin 19 antibody (29855-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Cytokeratin 19 Polyclonal antibody (29855-1-AP)

    IF Staining of HepG2 using 29855-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Cytokeratin 19 antibody (29855-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inBxPC-3 cells, HT-29 cells, HepG2 cells, MCF-7 cells, T-47D cells
Positive IHC detected inhuman breast cancer tissue, human colon tissue, human thyroid cancer tissue, human normal colon
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, HeLa cells, A549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29855-1-AP targets Cytokeratin 19 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cytokeratin 19 fusion protein Ag31469 相同性解析による交差性が予測される生物種
Full Name keratin 19
Calculated molecular weight44KD
Observed molecular weight40-44 kDa
GenBank accession numberNM_002276
Gene symbol Cytokeratin 19
Gene ID (NCBI) 3880
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 19 is a type I cytokeratin. It is a biochemical marker of skin stem cells in vivo and in vitro.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 19 antibody 29855-1-APDownload protocol
IHC protocol for Cytokeratin 19 antibody 29855-1-APDownload protocol
IF protocol for Cytokeratin 19 antibody 29855-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols