Cytokeratin 19 Monoclonal antibody, PBS Only

Various lysates were subjected to SDS PAGE followed by western blot with 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

MCF-7 cells were subjected to SDS PAGE followed by western blot with 60187-1-Ig (Cytokeratin 19 antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

WB result of KRT19 antibody (60187-1-IG, 1:10,000) with si-Control and si-KRT19 transfected HeLa cells. This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

HaCaT cells were subjected to SDS PAGE followed by western blot with 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

PC-3 cells were subjected to SDS PAGE followed by western blot with 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:51200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:51200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:12800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:12800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:12800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:12800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:12800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:12800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using Cytokeratin 19 antibody (60187-1-Ig, Clone: 3G1E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using Cytokeratin 19 antibody (60187-1-Ig, Clone: 3G1E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Cytokeratin 19 (60187-1-Ig, Clone:3G1E4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.