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Validation Data Gallery

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  • Western Blot (WB) analysis of COLO 320 cells using Cytokeratin 20 Monoclonal antibody (60183-1-Ig)

    WB analysis of COLO 320 using 60183-1-Ig (same clone as 60183-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 60183-1-Ig (Cytokeratin 20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using Cytokeratin 20 Monoclonal antibody (60183-1-Ig)

    WB analysis of COLO 320 using 60183-1-Ig (same clone as 60183-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 60183-1-Ig (Cytokeratin 20 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Cytokeratin 20 Monoclonal antibody (60183-1-Ig)

    IHC staining of human colon using 60183-1-Ig (same clone as 60183-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60183-1-Ig (Cytokeratin 20 antibody) at dilution of 1:51200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Cytokeratin 20 Monoclonal antibody (60183-1-Ig)

    IHC staining of human colon using 60183-1-Ig (same clone as 60183-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60183-1-Ig (Cytokeratin 20 antibody) at dilution of 1:51200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Cytokeratin 20 Monoclonal antibody (60183-1-Ig)

    IHC staining of human colon cancer using 60183-1-Ig (same clone as 60183-1-PBS)

    Immunohistochemical analysis of paraffin-embedded colon cancer slide using 60183-1-Ig (Cytokeratin 20 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Cytokeratin 20 Monoclonal antibody (60183-1-Ig)

    IHC staining of human colon cancer using 60183-1-Ig (same clone as 60183-1-PBS)

    Immunohistochemical analysis of paraffin-embedded colon cancer slide using 60183-1-Ig (Cytokeratin 20 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Cytokeratin 20 Monoclonal antibody (60183-1-Ig)

    IHC staining of human colon cancer using 60183-1-Ig (same clone as 60183-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60183-1-Ig (Cytokeratin 20 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using Cytokeratin 20 Monoclonal antibody (60183-1-Ig)

    IHC staining of human appendicitis using 60183-1-Ig (same clone as 60183-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60183-1-Ig (Cytokeratin 20 antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using Cytokeratin 20 Monoclonal antibody (60183-1-Ig)

    IHC staining of human appendicitis using 60183-1-Ig (same clone as 60183-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60183-1-Ig (Cytokeratin 20 antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Cytokeratin 20 Monoclonal antibody (60183-1-Ig)

    IHC staining of human breast cancer using 60183-1-Ig (same clone as 60183-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60183-1-Ig (Cytokeratin 20 antibody) at dilution of 1:6400 (under 10x lens) showing negative staining. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Cytokeratin 20 Monoclonal antibody (60183-1-Ig)

    IHC staining of human breast cancer using 60183-1-Ig (same clone as 60183-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60183-1-Ig (Cytokeratin 20 antibody) at dilution of 1:6400 (under 40x lens) showing negative staining.. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using Cytokeratin 20 Monoclonal antibody (60183-1-Ig)

    IHC staining of human urothelial carcinoma using 60183-1-Ig (same clone as 60183-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 60183-1-Ig (Cytokeratin 20 antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using Cytokeratin 20 Monoclonal antibody (60183-1-Ig)

    IHC staining of human urothelial carcinoma using 60183-1-Ig (same clone as 60183-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 60183-1-Ig (Cytokeratin 20 antibody) at dilution of 1:1600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human colon tissue using Cytokeratin 20 Monoclonal antibody (60183-1-Ig)

    IF Staining of human colon using 60183-1-Ig (same clone as 60183-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon tissue using Cytokeratin 20 antibody (60183-1-Ig, Clone: 4D10A4 ) at dilution of 1:400 and CoraLite®647-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Collagen Type III (N-terminal) antibody (22734-1-AP, green), CoraLite®594 CD68 antibody (CL594-25747, red). This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HT-29 cells using Cytokeratin 20 Monoclonal antibody (60183-1-Ig)

    IF Staining of HT-29 using 60183-1-Ig (same clone as 60183-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using Cytokeratin 20 antibody (60183-1-Ig, Clone: 4D10A4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.

Cytokeratin 20 Monoclonal antibody, PBS Only

Cytokeratin 20 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 60183-1-PBS
Clone No.4D10A4

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

KRT20, CK 20, CK20, CK-20, Cytokeratin-20

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60183-1-PBS targets Cytokeratin 20 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag11336

Product name: Recombinant human KRT20 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 74-424 aa of BC031559

Sequence: MQNLNDRLASYLEKVRTLEQSNSKLEVQIKQWYETNAPRAGRDYSAYYRQIEELRSQIKDAQLQNARCVLQIDNAKLAAEDFRLKYETERGIRLTVEADLQGLNKVFDDLTLHKTDLEIQIEELNKDLALLKKEHQEEVDGLHKHLGNTVNVEVDAAPGLNLGVIMNEMRQKYEVMAQKNLQEAKEQFERQTAVLQQQVTVNTEELKGTEVQLTELRRTSQSLEIELQSHLSMKESLEHTLEETKARYSSQLANLQSLLSSLEAQLMQIRSNMERQNNEYHILLDIKTRLEQEIATYRRLLEGEDVKTTEYQLSTLEERDIKKTRKIKTVVQEVVDGKVVSSEVKEVEENI

相同性解析による交差性が予測される生物種
Full Name keratin 20
Calculated molecular weight 424 aa, 48 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC031559
Gene Symbol Cytokeratin 20
Gene ID (NCBI) 54474
RRIDAB_10858399
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP35900
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18).Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa.