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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cytokeratin 20 Monoclonal antibody, PBS Only

Cytokeratin 20 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4D10A4

Cat no : 60183-1-PBS

Synonyms

KRT20, keratin 20, K20, Cytokeratin-20, CK-20

Validation Data Gallery

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  • Western Blot (WB) analysis of COLO 320 cells using Cytokeratin 20 Monoclonal antibody (60183-1-Ig)

    WB analysis of COLO 320 using 60183-1-Ig (same clone as 60183-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 60183-1-Ig (Cytokeratin 20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using Cytokeratin 20 Monoclonal antibody (60183-1-Ig)

    WB analysis of COLO 320 using 60183-1-Ig (same clone as 60183-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 60183-1-Ig (Cytokeratin 20 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Cytokeratin 20 Monoclonal antibody (60183-1-Ig)

    IHC staining of human colon using 60183-1-Ig (same clone as 60183-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60183-1-Ig (Cytokeratin 20 antibody) at dilution of 1:51200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Cytokeratin 20 Monoclonal antibody (60183-1-Ig)

    IHC staining of human colon using 60183-1-Ig (same clone as 60183-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60183-1-Ig (Cytokeratin 20 antibody) at dilution of 1:51200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Cytokeratin 20 Monoclonal antibody (60183-1-Ig)

    IHC staining of human colon cancer using 60183-1-Ig (same clone as 60183-1-PBS)

    Immunohistochemical analysis of paraffin-embedded colon cancer slide using 60183-1-Ig (Cytokeratin 20 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Cytokeratin 20 Monoclonal antibody (60183-1-Ig)

    IHC staining of human colon cancer using 60183-1-Ig (same clone as 60183-1-PBS)

    Immunohistochemical analysis of paraffin-embedded colon cancer slide using 60183-1-Ig (Cytokeratin 20 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Cytokeratin 20 Monoclonal antibody (60183-1-Ig)

    IHC staining of human breast cancer using 60183-1-Ig (same clone as 60183-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60183-1-Ig (Cytokeratin 20 antibody) at dilution of 1:6400 (under 10x lens) showing negative staining. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Cytokeratin 20 Monoclonal antibody (60183-1-Ig)

    IHC staining of human breast cancer using 60183-1-Ig (same clone as 60183-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60183-1-Ig (Cytokeratin 20 antibody) at dilution of 1:6400 (under 40x lens) showing negative staining.. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using Cytokeratin 20 Monoclonal antibody (60183-1-Ig)

    IHC staining of human urothelial carcinoma using 60183-1-Ig (same clone as 60183-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 60183-1-Ig (Cytokeratin 20 antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using Cytokeratin 20 Monoclonal antibody (60183-1-Ig)

    IHC staining of human urothelial carcinoma using 60183-1-Ig (same clone as 60183-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 60183-1-Ig (Cytokeratin 20 antibody) at dilution of 1:1600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using Cytokeratin 20 Monoclonal antibody (60183-1-Ig)

    IHC staining of human stomach using 60183-1-Ig (same clone as 60183-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 60183-1-Ig (Cytokeratin 20 antibody) at dilution of 1:6400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using Cytokeratin 20 Monoclonal antibody (60183-1-Ig)

    IHC staining of human stomach using 60183-1-Ig (same clone as 60183-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 60183-1-Ig (Cytokeratin 20 antibody) at dilution of 1:6400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using Cytokeratin 20 Monoclonal antibody (60183-1-Ig)

    IHC staining of human appendicitis using 60183-1-Ig (same clone as 60183-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60183-1-Ig (Cytokeratin 20 antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using Cytokeratin 20 Monoclonal antibody (60183-1-Ig)

    IHC staining of human appendicitis using 60183-1-Ig (same clone as 60183-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60183-1-Ig (Cytokeratin 20 antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human colon tissue using Cytokeratin 20 Monoclonal antibody (60183-1-Ig)

    IF Staining of human colon using 60183-1-Ig (same clone as 60183-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon tissue using Cytokeratin 20 antibody (60183-1-Ig, Clone: 4D10A4 ) at dilution of 1:400 and CoraLite®647-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Collagen Type III (N-terminal) antibody (22734-1-AP, green), CoraLite®594 CD68 antibody (CL594-25747, red). This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HT-29 cells using Cytokeratin 20 Monoclonal antibody (60183-1-Ig)

    IF Staining of HT-29 using 60183-1-Ig (same clone as 60183-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using Cytokeratin 20 antibody (60183-1-Ig, Clone: 4D10A4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60183-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60183-1-PBS targets Cytokeratin 20 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Cytokeratin 20 fusion protein Ag11336 相同性解析による交差性が予測される生物種
Full Name keratin 20
Calculated molecular weight 424 aa, 48 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC031559
Gene symbol Cytokeratin 20
Gene ID (NCBI) 54474
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18).Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa.