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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cytokeratin 5 Monoclonal antibody

Cytokeratin 5 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig and More (1)

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1A1C5

Cat no : 66727-1-Ig

Synonyms

CK 5, CK-5, keratin 5, KRT5, Cytokeratin,Keratin

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cytokeratin 5 Monoclonal antibody (66727-1-Ig)

    WB analysis using 66727-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skin tissue using Cytokeratin 5 Monoclonal antibody (66727-1-Ig)

    WB analysis of mouse skin using 66727-1-Ig

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SKOV-3 cells using Cytokeratin 5 Monoclonal antibody (66727-1-Ig)

    WB analysis of SKOV-3 using 66727-1-Ig

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skin tissue using Cytokeratin 5 Monoclonal antibody (66727-1-Ig)

    WB analysis of mouse skin using 66727-1-Ig

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using Cytokeratin 5 Monoclonal antibody (66727-1-Ig)

    WB analysis of MCF-7 using 66727-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using Cytokeratin 5 Monoclonal antibody (66727-1-Ig)

    WB analysis of A549 using 66727-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Cytokeratin 5 Monoclonal antibody (66727-1-Ig)

    IHC staining of human tonsillitis using 66727-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Cytokeratin 5 Monoclonal antibody (66727-1-Ig)

    IHC staining of human tonsillitis using 66727-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Cytokeratin 5 Monoclonal antibody (66727-1-Ig)

    IHC staining of human breast cancer using 66727-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Cytokeratin 5 Monoclonal antibody (66727-1-Ig)

    IHC staining of human breast cancer using 66727-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using Cytokeratin 5 Monoclonal antibody (66727-1-Ig)

    IHC staining of human oesophagus cancer using 66727-1-Ig

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using Cytokeratin 5 Monoclonal antibody (66727-1-Ig)

    IHC staining of human oesophagus cancer using 66727-1-Ig

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Cytokeratin 5 Monoclonal antibody (66727-1-Ig)

    IHC staining of human breast cancer using 66727-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:6000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Cytokeratin 5 Monoclonal antibody (66727-1-Ig)

    IHC staining of human breast cancer using 66727-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:6000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast hyperplasia tissue using Cytokeratin 5 Monoclonal antibody (66727-1-Ig)

    IHC staining of human breast hyperplasia using 66727-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:6000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast hyperplasia tissue using Cytokeratin 5 Monoclonal antibody (66727-1-Ig)

    IHC staining of human breast hyperplasia using 66727-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:6000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Cytokeratin 5 Monoclonal antibody (66727-1-Ig)

    IHC staining of human lung cancer using 66727-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:6000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Cytokeratin 5 Monoclonal antibody (66727-1-Ig)

    IHC staining of human lung cancer using 66727-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:6000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast hyperplasia tissue using Cytokeratin 5 Monoclonal antibody (66727-1-Ig)

    IHC staining of human breast hyperplasia using 66727-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:6000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast hyperplasia tissue using Cytokeratin 5 Monoclonal antibody (66727-1-Ig)

    IHC staining of human breast hyperplasia using 66727-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:6000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Cytokeratin 5 Monoclonal antibody (66727-1-Ig)

    IHC staining of human breast cancer using 66727-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:6000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using Cytokeratin 5 Monoclonal antibody (66727-1-Ig)

    IHC staining of human skin cancer using 66727-1-Ig

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using Cytokeratin 5 Monoclonal antibody (66727-1-Ig)

    IHC staining of human skin cancer using 66727-1-Ig

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using Cytokeratin 5 Monoclonal antibody (66727-1-Ig)

    IHC staining of human skin cancer using 66727-1-Ig

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using Cytokeratin 5 Monoclonal antibody (66727-1-Ig)

    IHC staining of human liver using 66727-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:10000 (under 10x lens) showing negative staining. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Cytokeratin 5 Monoclonal antibody (66727-1-Ig)

    IHC staining of human breast cancer using 66727-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:6000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using Cytokeratin 5 Monoclonal antibody (66727-1-Ig)

    IHC staining of human skin cancer using 66727-1-Ig

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using Cytokeratin 5 Monoclonal antibody (66727-1-Ig)

    IHC staining of human liver using 66727-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:10000 (under 40x lens) showing negative staining. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using Cytokeratin 5 Monoclonal antibody (66727-1-Ig)

    IHC staining of human cervical cancer using 66727-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:3000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using Cytokeratin 5 Monoclonal antibody (66727-1-Ig)

    IHC staining of human cervical cancer using 66727-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:3000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human oesophagus cancer tissue using Cytokeratin 5 Monoclonal antibody (66727-1-Ig)

    IF Staining of human oesophagus cancer using 66727-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human oesophagus cancer tissue using Cytokeratin 5 antibody (66727-1-Ig, Clone: 1A1C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human oesophagus cancer tissue using Cytokeratin 5 Monoclonal antibody (66727-1-Ig)

    IF Staining of human oesophagus cancer using 66727-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human oesophagus cancer tissue using Cytokeratin 5 antibody (66727-1-Ig, Clone: 1A1C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human prostate cancer tissue using Cytokeratin 5 Monoclonal antibody (66727-1-Ig)

    IF Staining of human prostate cancer using 66727-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using Cytokeratin 5 antibody (66727-1-Ig, Clone: 1A1C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human prostate cancer tissue using Cytokeratin 5 Monoclonal antibody (66727-1-Ig)

    IF Staining of human prostate cancer using 66727-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using Cytokeratin 5 antibody (66727-1-Ig, Clone: 1A1C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human oesophagus cancer tissue using Cytokeratin 5 Monoclonal antibody (66727-1-Ig)

    IF Staining of human oesophagus cancer using 66727-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human oesophagus cancer tissue using Cytokeratin 5 antibody (66727-1-Ig, Clone: 1A1C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human oesophagus cancer tissue using Cytokeratin 5 Monoclonal antibody (66727-1-Ig)

    IF Staining of human oesophagus cancer using 66727-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human oesophagus cancer tissue using Cytokeratin 5 antibody (66727-1-Ig, Clone: 1A1C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using Cytokeratin 5 Monoclonal antibody (66727-1-Ig)

    IF Staining of A431 using 66727-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Cytokeratin 5 antibody (66727-1-Ig, Clone: 1A1C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inHaCaT cells, SKOV-3 cells, mouse skin tissue, MCF-7 cells, A549 cells, A-253 cells, A431 cells, human saliva, pig skin tissue
Positive IHC detected inhuman tonsillitis tissue, human breast cancer tissue, human breast hyperplasia tissue, human lung cancer tissue, human oesophagus cancer tissue, human cervical cancer tissue, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman oesophagus cancer tissue, human prostate cancer tissue
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:5000-1:20000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66727-1-Ig targets Cytokeratin 5 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Reactivity human, mouse, pig
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Cytokeratin 5 fusion protein Ag24184 相同性解析による交差性が予測される生物種
Full Name keratin 5
Calculated molecular weight 590 aa, 62 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC024292
Gene symbol Cytokeratin 5
Gene ID (NCBI) 3852
RRIDAB_2882077
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18). Keratin 5, one 58-kD type II keratin, is coexpressed with a 50-kD keratin 14 in stratified squamous epithelia. Keratin 5, one 58-kD type II keratin, is coexpressed with a 50-kD tyK14 in stratified squamous epithelia.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 5 antibody 66727-1-IgDownload protocol
IHC protocol for Cytokeratin 5 antibody 66727-1-IgDownload protocol
IF protocol for Cytokeratin 5 antibody 66727-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIF

Cell Mol Biol Lett

The role of NPY2R/NFATc1/DYRK1A regulatory axis in sebaceous glands for sebum synthesis

Authors - Tao Yang
View Article
mouseIHC

Front Genet

The Unique Genetic and Histological Characteristics of DMBA-Induced Mammary Tumors in an Organoid-Based Carcinogenesis Model.

Authors - Mie Naruse
View Article
humanWB

PLoS One

Construction of ceRNA network to identify the lncRNA and mRNA related to non-small cell lung cancer.

Authors - Kui Xiao
View Article
humanWB

Cell Death Dis

SOX1 promotes differentiation of nasopharyngeal carcinoma cells by activating retinoid metabolic pathway.

Authors - Xin-Xing Lei
View Article
humanWB

Cell Death Discov

TRIM29 hypermethylation drives esophageal cancer progression via suppression of ZNF750

Authors - Qiyi Yi
View Article
mouseIHC

Immunobiology

High expression of NF-κB inducing kinase in the bulge region of hair follicle induces tumor

Authors - Weisong Duan
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Emma (Verified Customer) (11-29-2021)

Used by IF on FFPE prostate tissue. Tris-EFTA antigen retrieval.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Prostate
Cytokeratin 5 Antibody Immunofluorescence validation (1:100 dilution) in Prostate (Cat no:66727-1-Ig)