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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cytokeratin 6A Monoclonal antibody (68294-1-Ig)

    WB analysis using 68294-1-Ig (same clone as 68294-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68294-1-Ig (Cytokeratin 6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68294-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using Cytokeratin 6A Monoclonal antibody (68294-1-Ig)

    IHC staining of human cervical cancer using 68294-1-Ig (same clone as 68294-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 68294-1-Ig (Cytokeratin 6 antibody) at dilution of 1:2300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68294-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using Cytokeratin 6A Monoclonal antibody (68294-1-Ig)

    IHC staining of human cervical cancer using 68294-1-Ig (same clone as 68294-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 68294-1-Ig (Cytokeratin 6 antibody) at dilution of 1:2300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68294-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using Cytokeratin 6A Monoclonal antibody (68294-1-Ig)

    IHC staining of human cervical cancer using 68294-1-Ig (same clone as 68294-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 68294-1-Ig (Cytokeratin 6 antibody) at dilution of 1:2300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68294-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using Cytokeratin 6A Monoclonal antibody (68294-1-Ig)

    IHC staining of human cervical cancer using 68294-1-Ig (same clone as 68294-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 68294-1-Ig (Cytokeratin 6 antibody) at dilution of 1:2300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68294-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using Cytokeratin 6A Monoclonal antibody (68294-1-Ig)

    IHC staining of human oesophagus cancer using 68294-1-Ig (same clone as 68294-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 68294-1-Ig (Cytokeratin 6 antibody) at dilution of 1:2300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68294-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using Cytokeratin 6A Monoclonal antibody (68294-1-Ig)

    IHC staining of human oesophagus cancer using 68294-1-Ig (same clone as 68294-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 68294-1-Ig (Cytokeratin 6 antibody) at dilution of 1:2300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68294-1-PBS in a different storage buffer formulation.

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Cytokeratin 6A Monoclonal antibody, PBS Only

Cytokeratin 6A Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 68294-1-PBS
Clone No.1G1D7

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

CK 6A, CK 6D, CK6, CK6A, CK6C, CK6D, Cytokeratin 6, Cytokeratin 6A, Cytokeratin 6D, Hom s 5, K6A, K6C, K6D, keratin 6A, KRT conserve, KRT6, KRT6A, KRT6C, KRT6D, Type II keratin Kb6

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68294-1-PBS targets Cytokeratin 6A in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Cytokeratin 6A fusion protein Ag0882 相同性解析による交差性が予測される生物種
Full Name keratin 6A
Calculated molecular weight 60 kDa
Observed molecular weight60 kDa
GenBank accession numberBC008807
Gene Symbol Cytokeratin 6A
Gene ID (NCBI) 3853
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP02538
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 6 is a type II keratin. It is used as a marker for epidermal hyperproliferation and differentiation.