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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cytokeratin 6A Monoclonal antibody, PBS Only (Capture)

Cytokeratin 6A Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2H6A11

Cat no : 66685-1-PBS

Synonyms

Cytokeratin 6, KRT6A, CK-6A, CK6A, CK-6

Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using Cytokeratin 6A Monoclonal antibody (66685-1-Ig)

    WB analysis of A431 using 66685-1-Ig (same clone as 66685-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 66685-1-Ig (KRT6A antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66685-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using Cytokeratin 6A Monoclonal antibody (66685-1-Ig)

    IHC staining of human cervical cancer using 66685-1-Ig (same clone as 66685-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66685-1-Ig (Cytokeratin 6A antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66685-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using Cytokeratin 6A Monoclonal antibody (66685-1-Ig)

    IHC staining of human cervical cancer using 66685-1-Ig (same clone as 66685-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66685-1-Ig (Cytokeratin 6A antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66685-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50382-1

    Cytometric bead array standard curve of MP50382-1

    Cytometric bead array standard curve of MP50382-1, KRT6A Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66685-1-PBS. Detection antibody: 68294-2-PBS. Standard:Ag0882. Range: 0.098-100 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66685-1-PBS targets Cytokeratin 6A as part of a matched antibody pair:

MP50382-1: 66685-1-PBS capture and 68294-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Cytokeratin 6A fusion protein Ag0882 相同性解析による交差性が予測される生物種
Full Name keratin 6A
Calculated molecular weight 60 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC008807
Gene symbol Cytokeratin 6A
Gene ID (NCBI) 3853
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 6 is a type II keratin. It is used as marker for epidermal hyperproliferation and differentiation. Three are three keratin 6 isoforms: ketatin 6A, 6B and 6C. They share more than 99% identical DNA sequence.