Validation Data Gallery

Filter: AllWBIHCIF

WB analysis using 66483-1-Ig (same clone as 66483-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66483-1-Ig (CK7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66483-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 66483-1-Ig (same clone as 66483-1-PBS)
WB result of CK7 antibody (66483-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CK7 transfected HeLa cells. This data was developed using the same antibody clone with 66483-1-PBS in a different storage buffer formulation.
WB analysis of A431 using 66483-1-Ig (same clone as 66483-1-PBS)
A431 cells were subjected to SDS PAGE followed by western blot with 66483-1-Ig (CK7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66483-1-PBS in a different storage buffer formulation.
WB analysis of T-47D using 66483-1-Ig (same clone as 66483-1-PBS)
T-47D cells were subjected to SDS PAGE followed by western blot with 66483-1-Ig (CK7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66483-1-PBS in a different storage buffer formulation.
WB analysis of PC-3 using 66483-1-Ig (same clone as 66483-1-PBS)
PC-3 cells were subjected to SDS PAGE followed by western blot with 66483-1-Ig (CK7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66483-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 66483-1-Ig (same clone as 66483-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66483-1-Ig (CK7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66483-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 66483-1-Ig (same clone as 66483-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66483-1-Ig (CK7 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66483-1-PBS in a different storage buffer formulation.
IHC staining of human ovary tumor using 66483-1-Ig (same clone as 66483-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66483-1-Ig (CK7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66483-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 66483-1-Ig (same clone as 66483-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66483-1-Ig (Cytokeratin 7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66483-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 66483-1-Ig (same clone as 66483-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66483-1-Ig (Cytokeratin 7 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66483-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 66483-1-Ig (same clone as 66483-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66483-1-Ig (Cytokeratin 7 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66483-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 66483-1-Ig (same clone as 66483-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66483-1-Ig (Cytokeratin 7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66483-1-PBS in a different storage buffer formulation.
IHC staining of human ovary tumor using 66483-1-Ig (same clone as 66483-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66483-1-Ig (CK7 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66483-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas using 66483-1-Ig (same clone as 66483-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66483-1-Ig (Cytokeratin 7 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66483-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas using 66483-1-Ig (same clone as 66483-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66483-1-Ig (Cytokeratin 7 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66483-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 66483-1-Ig (same clone as 66483-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66483-1-Ig (Cytokeratin 7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66483-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 66483-1-Ig (same clone as 66483-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66483-1-Ig (Cytokeratin 7 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66483-1-PBS in a different storage buffer formulation.
IF Staining of human prostate cancer using 66483-1-Ig (same clone as 66483-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using Cytokeratin 7 antibody (66483-1-Ig, Clone: 2E1G5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66483-1-PBS in a different storage buffer formulation.
IF Staining of human prostate cancer using 66483-1-Ig (same clone as 66483-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using Cytokeratin 7 antibody (66483-1-Ig, Clone: 2E1G5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66483-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

Cytokeratin 7 Monoclonal antibody, PBS Only

Cytokeratin 7 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 66483-1-PBS

Clone No.2E1G5

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Cell and organelle markers, CK 7, CK7, Cytokeratin 7, Cytoskeleton Marker, K2C7, K7, keratin 7, KRT7, Sarcolectin, SCL, Type II keratin Kb7

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66483-1-PBS targets Cytokeratin 7 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity	Human
Host / Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Immunogen	Cytokeratin 7 fusion protein Ag7895 相同性解析による交差性が予測される生物種
Full Name	keratin 7
Calculated molecular weight	469 aa, 51 kDa
Observed molecular weight	51 kDa
GenBank accession number	BC002700
Gene Symbol	Cytokeratin 7
Gene ID (NCBI)	3855
RRID	AB_2881849
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P08729
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT7, also named as cytokeratin 7, is one member of type II basic cytokeratin. It is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels, and their neoplasms. KRT7 is marker of epithelial tissues, but not present in carcinomas of stratified squamous cell origin.This antibody is specifically against KRT7.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 66483-1-Ig (same clone as 66483-1-PBS)

WB analysis of HeLa using 66483-1-Ig (same clone as 66483-1-PBS)

WB analysis of A431 using 66483-1-Ig (same clone as 66483-1-PBS)

WB analysis of T-47D using 66483-1-Ig (same clone as 66483-1-PBS)

WB analysis of PC-3 using 66483-1-Ig (same clone as 66483-1-PBS)

IHC staining of human lung cancer using 66483-1-Ig (same clone as 66483-1-PBS)

IHC staining of human lung cancer using 66483-1-Ig (same clone as 66483-1-PBS)

IHC staining of human ovary tumor using 66483-1-Ig (same clone as 66483-1-PBS)

IHC staining of human breast cancer using 66483-1-Ig (same clone as 66483-1-PBS)

IHC staining of human breast cancer using 66483-1-Ig (same clone as 66483-1-PBS)

IHC staining of human breast cancer using 66483-1-Ig (same clone as 66483-1-PBS)

IHC staining of human breast cancer using 66483-1-Ig (same clone as 66483-1-PBS)

IHC staining of human ovary tumor using 66483-1-Ig (same clone as 66483-1-PBS)

IHC staining of human pancreas using 66483-1-Ig (same clone as 66483-1-PBS)

IHC staining of human pancreas using 66483-1-Ig (same clone as 66483-1-PBS)

IHC staining of human kidney using 66483-1-Ig (same clone as 66483-1-PBS)

IHC staining of human kidney using 66483-1-Ig (same clone as 66483-1-PBS)

IF Staining of human prostate cancer using 66483-1-Ig (same clone as 66483-1-PBS)

IF Staining of human prostate cancer using 66483-1-Ig (same clone as 66483-1-PBS)

Cytokeratin 7 Monoclonal antibody, PBS Only

Cytokeratin 7 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

-/ -

Tested Applications

Recommended dilution

Product Information

Background Information