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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cytokeratin 7 Monoclonal antibody, PBS Only

Cytokeratin 7 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3G2H1

Cat no : 68296-1-PBS

Synonyms

CK 7, CK7, Cytokeratin 7, K2C7, K7, keratin 7, KRT7, Sarcolectin, SCL, Type II keratin Kb7

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cytokeratin 7 Monoclonal antibody (68296-1-Ig)

    WB analysis using 68296-1-Ig (same clone as 68296-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68296-1-Ig (Cytokeratin 7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68296-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Cytokeratin 7 Monoclonal antibody (68296-1-Ig)

    WB analysis using 68296-1-Ig (same clone as 68296-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68296-1-Ig (Cytokeratin 7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68296-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Cytokeratin 7 Monoclonal antibody (68296-1-Ig)

    IHC staining of human lung cancer using 68296-1-Ig (same clone as 68296-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68296-1-Ig (Cytokeratin 7 antibody) at dilution of 1:8300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68296-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Cytokeratin 7 Monoclonal antibody (68296-1-Ig)

    IHC staining of human lung cancer using 68296-1-Ig (same clone as 68296-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68296-1-Ig (Cytokeratin 7 antibody) at dilution of 1:8300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68296-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using Cytokeratin 7 Monoclonal antibody (68296-1-Ig)

    IHC staining of human endometrial cancer using 68296-1-Ig (same clone as 68296-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 68296-1-Ig (Cytokeratin 7 antibody) at dilution of 1:8300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68296-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using Cytokeratin 7 Monoclonal antibody (68296-1-Ig)

    IHC staining of human endometrial cancer using 68296-1-Ig (same clone as 68296-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 68296-1-Ig (Cytokeratin 7 antibody) at dilution of 1:8300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68296-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using Cytokeratin 7 Monoclonal antibody (68296-1-Ig)

    IHC staining of human ovary tumor using 68296-1-Ig (same clone as 68296-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 68296-1-Ig (Cytokeratin 7 antibody) at dilution of 1:8300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68296-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using Cytokeratin 7 Monoclonal antibody (68296-1-Ig)

    IHC staining of human ovary tumor using 68296-1-Ig (same clone as 68296-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 68296-1-Ig (Cytokeratin 7 antibody) at dilution of 1:8300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68296-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Cytokeratin 7 Monoclonal antibody (68296-1-Ig)

    IHC staining of human pancreas cancer using 68296-1-Ig (same clone as 68296-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 68296-1-Ig (Cytokeratin 7 antibody) at dilution of 1:8300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68296-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Cytokeratin 7 Monoclonal antibody (68296-1-Ig)

    IHC staining of human pancreas cancer using 68296-1-Ig (same clone as 68296-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 68296-1-Ig (Cytokeratin 7 antibody) at dilution of 1:8300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68296-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Cytokeratin 7 Monoclonal antibody (68296-1-Ig)

    IHC staining of human lung cancer using 68296-1-Ig (same clone as 68296-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68296-1-Ig (Cytokeratin 7 antibody) at dilution of 1:8300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68296-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Cytokeratin 7 Monoclonal antibody (68296-1-Ig)

    IHC staining of human lung cancer using 68296-1-Ig (same clone as 68296-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68296-1-Ig (Cytokeratin 7 antibody) at dilution of 1:8300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68296-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68296-1-PBS targets Cytokeratin 7 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Cytokeratin 7 fusion protein Ag7895 相同性解析による交差性が予測される生物種
Full Name keratin 7
Calculated molecular weight 469 aa, 51 kDa
GenBank accession numberBC002700
Gene symbol Cytokeratin 7
Gene ID (NCBI) 3855
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT7, also named cytokeratin 7, is one member of type II basic cytokeratin. It is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels, and their neoplasms. KRT7 is a marker of epithelial tissues, but not present in carcinomas of stratified squamous cell origin.