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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using Cytokeratin 7-specific Polyclonal antibody (16857-1-AP)

    WB analysis of HepG2 using 16857-1-AP (same clone as 16857-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16857-1-AP (KRT7-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16857-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Cytokeratin 7-specific Polyclonal antibody (16857-1-AP)

    IHC staining of human lung cancer using 16857-1-AP (same clone as 16857-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16857-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16857-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Cytokeratin 7-specific Polyclonal antibody (16857-1-AP)

    IHC staining of human lung cancer using 16857-1-AP (same clone as 16857-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16857-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16857-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Cytokeratin 7-specific Polyclonal antibody (16857-1-AP)

    IHC staining of human breast cancer using 16857-1-AP (same clone as 16857-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16857-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16857-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Cytokeratin 7-specific Polyclonal antibody (16857-1-AP)

    IHC staining of human breast cancer using 16857-1-AP (same clone as 16857-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16857-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16857-1-PBS in a different storage buffer formulation.

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Cytokeratin 7-specific Polyclonal antibody, PBS Only

Cytokeratin 7-specific Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 16857-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16857-1-PBS targets Cytokeratin 7-specific in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name keratin 7
Calculated molecular weight 51 kDa
Observed molecular weight 51 kDa
GenBank accession numberNM_005556
Gene Symbol Cytokeratin 7
Gene ID (NCBI) 3855
RRIDAB_2134446
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP08729
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT7, also named as cytokeratin 7, is one member of type II basic cytokeratin. It is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels, and their neoplasms. KRT7 is marker of epithelial tissues, but not present in carcinomas of stratified squamous cell origin.This antibody is specifically against KRT7.