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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cytokeratin 8 Monoclonal antibody, PBS Only (Capture)

Cytokeratin 8 Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F2H5

Cat no : 68627-1-PBS

Synonyms

KRT8, Cytokeratin,Keratin, CK-8, CK8, CK 8

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cytokeratin 8 Monoclonal antibody (68627-1-Ig)

    WB analysis using 68627-1-Ig (same clone as 68627-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68627-1-Ig (KRT8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68627-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using Cytokeratin 8 Monoclonal antibody (68627-1-Ig)

    WB analysis of A549 using 68627-1-Ig (same clone as 68627-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 68627-1-Ig (KRT8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68627-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat colon tissue using Cytokeratin 8 Monoclonal antibody (68627-1-Ig)

    WB analysis of rat colon using 68627-1-Ig (same clone as 68627-1-PBS)

    rat colon tissue were subjected to SDS PAGE followed by western blot with 68627-1-Ig (KRT8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68627-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse colon tissue using Cytokeratin 8 Monoclonal antibody (68627-1-Ig)

    WB analysis of mouse colon using 68627-1-Ig (same clone as 68627-1-PBS)

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 68627-1-Ig (KRT8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68627-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Cytokeratin 8 Monoclonal antibody (68627-1-Ig)

    IHC staining of human colon using 68627-1-Ig (same clone as 68627-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 68627-1-Ig (Cytokeratin 8 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68627-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Cytokeratin 8 Monoclonal antibody (68627-1-Ig)

    IHC staining of human tonsillitis using 68627-1-Ig (same clone as 68627-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68627-1-Ig (Cytokeratin 8 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68627-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Cytokeratin 8 Monoclonal antibody (68627-1-Ig)

    IHC staining of human colon using 68627-1-Ig (same clone as 68627-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 68627-1-Ig (Cytokeratin 8 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68627-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Cytokeratin 8 Monoclonal antibody (68627-1-Ig)

    IHC staining of human tonsillitis using 68627-1-Ig (same clone as 68627-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68627-1-Ig (Cytokeratin 8 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68627-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51080-1

    Cytometric bead array standard curve of MP51080-1

    Cytometric bead array standard curve of MP51080-1, Cytokeratin 8 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68627-1-PBS. Detection antibody: 68627-2-PBS. Standard:Ag0488. Range: 0.098-100 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68627-1-PBS targets Cytokeratin 8 as part of a matched antibody pair:

MP51080-1: 68627-1-PBS capture and 68627-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Cytokeratin 8 fusion protein Ag0488 相同性解析による交差性が予測される生物種
Full Name keratin 8
Calculated molecular weight 54 kDa
Observed molecular weight50-52 kDa
GenBank accession numberBC000654
Gene symbol Cytokeratin 8
Gene ID (NCBI) 3856
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT8 is often paired with keratin 18 in vivo.