• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cytokeratin 8 Monoclonal antibody, PBS Only

Cytokeratin 8 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F2H5

Cat no : 68627-1-PBS

Synonyms

CARD2, CK 8, CK8, CYK8, Cytokeratin 8, K2C8, K8, keratin 8, KO, KRT8, Type II keratin Kb8

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using Cytokeratin 8 Monoclonal antibody (68627-1-Ig)

    WB analysis using 68627-1-Ig (same clone as 68627-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68627-1-Ig (KRT8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68627-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using Cytokeratin 8 Monoclonal antibody (68627-1-Ig)

    WB analysis of A549 using 68627-1-Ig (same clone as 68627-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 68627-1-Ig (KRT8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68627-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat colon tissue using Cytokeratin 8 Monoclonal antibody (68627-1-Ig)

    WB analysis of rat colon using 68627-1-Ig (same clone as 68627-1-PBS)

    rat colon tissue were subjected to SDS PAGE followed by western blot with 68627-1-Ig (KRT8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68627-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse colon tissue using Cytokeratin 8 Monoclonal antibody (68627-1-Ig)

    WB analysis of mouse colon using 68627-1-Ig (same clone as 68627-1-PBS)

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 68627-1-Ig (KRT8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68627-1-PBS in a different storage buffer formulation.

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68627-1-PBS targets Cytokeratin 8 in WB, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Cytokeratin 8 fusion protein Ag0488 相同性解析による交差性が予測される生物種
Full Name keratin 8
Calculated molecular weight 54 kDa
Observed molecular weight50-52 kDa
GenBank accession numberBC000654
Gene symbol Cytokeratin 8
Gene ID (NCBI) 3856
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT8 is often paired with keratin 18 in vivo.