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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cytokeratin 8 Polyclonal antibody

Cytokeratin 8 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 27105-1-AP

Synonyms

Cytokeratin 8-specific, KRT8, Cytokeratin,Keratin, CK-8, CK8

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cytokeratin 8-specific Polyclonal antibody (27105-1-AP)

    WB analysis using 27105-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27105-1-AP (Cytokeratin 8-specific antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Cytokeratin 8-specific Polyclonal antibody (27105-1-AP)

    IHC staining of human tonsillitis using 27105-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27105-1-AP (Cytokeratin 8-specific antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Cytokeratin 8-specific Polyclonal antibody (27105-1-AP)

    IHC staining of human tonsillitis using 27105-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27105-1-AP (Cytokeratin 8-specific antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brown disease using Cytokeratin 8-specific Polyclonal antibody (27105-1-AP)

    IHC staining of human brown disease using 27105-1-AP

    Immunohistochemical analysis of paraffin-embedded human brown disease slide using 27105-1-AP (Cytokeratin 8-specific antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brown disease using Cytokeratin 8-specific Polyclonal antibody (27105-1-AP)

    IHC staining of human brown disease using 27105-1-AP

    Immunohistochemical analysis of paraffin-embedded human brown disease slide using 27105-1-AP (Cytokeratin 8-specific antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using Cytokeratin 8-specific Polyclonal antibody (27105-1-AP)

    IHC staining of human colon using 27105-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27105-1-AP (Cytokeratin 8-specific antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using Cytokeratin 8-specific Polyclonal antibody (27105-1-AP)

    IHC staining of human colon using 27105-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27105-1-AP (Cytokeratin 8-specific antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using Cytokeratin 8-specific Polyclonal antibody (27105-1-AP)

    IHC staining of human colon using 27105-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27105-1-AP (Cytokeratin 8 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using Cytokeratin 8-specific Polyclonal antibody (27105-1-AP)

    IHC staining of human colon using 27105-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27105-1-AP (Cytokeratin 8 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat skin tissue using Cytokeratin 8-specific Polyclonal antibody (27105-1-AP)

    IHC staining of rat skin using 27105-1-AP

    Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 27105-1-AP (Cytokeratin 8-specific antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat skin tissue using Cytokeratin 8-specific Polyclonal antibody (27105-1-AP)

    IHC staining of rat skin using 27105-1-AP

    Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 27105-1-AP (Cytokeratin 8-specific antibody) at dilution of 1:4000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skin tissue using Cytokeratin 8-specific Polyclonal antibody (27105-1-AP)

    IHC staining of mouse skin using 27105-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 27105-1-AP (Cytokeratin 8-specific antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skin tissue using Cytokeratin 8-specific Polyclonal antibody (27105-1-AP)

    IHC staining of mouse skin using 27105-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 27105-1-AP (Cytokeratin 8-specific antibody) at dilution of 1:4000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Cytokeratin 8-specific Polyclonal antibody (27105-1-AP)

    IF Staining of HepG2 using 27105-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Cytokeratin 8-specific antibody (27105-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inhuman tonsillitis tissue, human colon tissue, mouse skin tissue, rat skin tissue, human brown disease
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27105-1-AP targets Cytokeratin 8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cytokeratin 8 fusion protein Ag25844 相同性解析による交差性が予測される生物種
Full Name keratin 8
Calculated molecular weight 54 kDa
Observed molecular weight52 kDa
GenBank accession numberBC008200
Gene symbol Cytokeratin 8
Gene ID (NCBI) 3856
RRIDAB_2918117
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT8 is often paired with keratin 18 in vivo.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 8 antibody 27105-1-APDownload protocol
IHC protocol for Cytokeratin 8 antibody 27105-1-APDownload protocol
IF protocol for Cytokeratin 8 antibody 27105-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Transl Lung Cancer Res

Identification of lysosomal genes associated with prognosis in lung adenocarcinoma

Authors - Houqiang Li
View Article
humanIHC

Environ Toxicol

Multidimensional progressive single-cell sequencing reveals cell microenvironment composition and cancer heterogeneity in lung cancer

Authors - Hua Chen
View Article
humanWB,IHC

J Cancer

Identification and validation of a novel apoptosis-related prognostic risk score model for lung adenocarcinoma

Authors - Yan Wang
View Article