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KD/KO Validated

D2HGDH Polyclonal antibody

Name: D2HGDH Polyclonal antibody
Brand: Proteintech
SKU: 13895-1-AP

D2HGDH Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 13895-1-AP

Synonyms

EC:1.1.99.39, D-2-hydroxyglutarate dehydrogenase, mitochondrial, D2HGD

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of mouse kidney using 13895-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13895-1-AP (D2HGDH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 13895-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 13895-1-AP (D2HGDH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 13895-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13895-1-AP (D2HGDH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 13895-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 13895-1-AP (D2HGDH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat liver using 13895-1-AP
rat liver tissue were subjected to SDS PAGE followed by western blot with 13895-1-AP (D2HGDH Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 13895-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 13895-1-AP (D2HGDH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse liver tissue using 13895-1-AP
IP result of anti-D2HGDH (13895-1-AP for IP and Detection) with mouse liver tissue lysate.
IP experiment of mouse liver using 13895-1-AP
IP result of anti-D2HGDH (IP:13895-1-AP, 3ug; Detection:13895-1-AP 1:500) with mouse liver tissue lysate 5000ug.
IHC staining of human liver cancer using 13895-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13895-1-AP (D2HGDH Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 13895-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13895-1-AP (D2HGDH Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 13895-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 13895-1-AP (D2HGDH antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 13895-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse kidney tissue, human liver tissue, human heart tissue, rat liver tissue, mouse liver tissue
Positive IP detected in	mouse liver tissue
Positive IHC detected in	human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 10 publications below
IHC	See 2 publications below
IF	See 2 publications below

Product Information

13895-1-AP targets D2HGDH in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	D2HGDH fusion protein Ag4857 相同性解析による交差性が予測される生物種
Full Name	D-2-hydroxyglutarate dehydrogenase
Calculated molecular weight	521 aa, 56 kDa
Observed molecular weight	56 kDa
GenBank accession number	BC036604
Gene symbol	D2HGDH
Gene ID (NCBI)	728294
RRID	AB_2089330
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

D2HGDH(D-2-hydroxyglutarate dehydrogenase, mitochondrial) is also named as D2HGD and belongs to the FAD-binding oxidoreductase/transferase type 4 family.It catalyzes the oxidation of D-2-hydroxyglutarate to alpha-ketoglutarate.Defects in D2HGDH are the cause of D-2-hydroxyglutaric aciduria type 1 (D2HGA1).It has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for D2HGDH antibody 13895-1-AP	Download protocol
IHC protocol for D2HGDH antibody 13895-1-AP	Download protocol
IF protocol for D2HGDH antibody 13895-1-AP	Download protocol
IP protocol for D2HGDH antibody 13895-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Metab Hypoxia Induces Production of L-2-Hydroxyglutarate. Authors - Andrew M Intlekofer KD Validated View Article
human	WB	Cell Metab Hypoxia-Mediated Increases in L-2-hydroxyglutarate Coordinate the Metabolic Response to Reductive Stress. Authors - William M Oldham KD Validated View Article
human	WB, IF	Nat Commun D2HGDH regulates alpha-ketoglutarate levels and dioxygenase function by modulating IDH2. Authors - An-Ping Lin View Article
human	WB	Cell Chem Biol MYC Regulation of D2HGDH and L2HGDH Influences the Epigenome and Epitranscriptome. Authors - ZhiJun Qiu KO Validated View Article
human	WB	Leukemia MYC, mitochondrial metabolism and O-GlcNAcylation converge to modulate the activity and subcellular localization of DNA and RNA demethylases. Authors - An-Ping Lin View Article
	WB	Oncotarget D-2-hydroxyglutarate is essential for maintaining oncogenic property of mutant IDH-containing cancer cells but dispensable for cell growth. Authors - Shenghong Ma View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

D2HGDH Polyclonal antibody

D2HGDH Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of mouse kidney using 13895-1-AP

WB analysis of human heart using 13895-1-AP

WB analysis of mouse kidney using 13895-1-AP

WB analysis of mouse liver using 13895-1-AP

WB analysis of rat liver using 13895-1-AP

WB analysis of human liver using 13895-1-AP

IP experiment of mouse liver tissue using 13895-1-AP

IP experiment of mouse liver using 13895-1-AP

IHC staining of human liver cancer using 13895-1-AP

IHC staining of human liver cancer using 13895-1-AP

IF Staining of HepG2 using 13895-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Hypoxia Induces Production of L-2-Hydroxyglutarate.

Hypoxia-Mediated Increases in L-2-hydroxyglutarate Coordinate the Metabolic Response to Reductive Stress.

D2HGDH regulates alpha-ketoglutarate levels and dioxygenase function by modulating IDH2.

MYC Regulation of D2HGDH and L2HGDH Influences the Epigenome and Epitranscriptome.

MYC, mitochondrial metabolism and O-GlcNAcylation converge to modulate the activity and subcellular localization of DNA and RNA demethylases.

D-2-hydroxyglutarate is essential for maintaining oncogenic property of mutant IDH-containing cancer cells but dispensable for cell growth.