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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

D2HGDH Monoclonal antibody, PBS Only

D2HGDH Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

rat, mouse, human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E9B3

Cat no : 66364-1-PBS

Synonyms

EC:1.1.99.39, D-2-hydroxyglutarate dehydrogenase, mitochondrial, D2HGD, 1E9B3

Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using D2HGDH Monoclonal antibody (66364-1-Ig)

    WB analysis of NIH/3T3 using 66364-1-Ig (same clone as 66364-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66364-1-Ig (D2HGDH Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66364-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of C6 cells using D2HGDH Monoclonal antibody (66364-1-Ig)

    WB analysis of C6 using 66364-1-Ig (same clone as 66364-1-PBS)

    C6 cells were subjected to SDS PAGE followed by western blot with 66364-1-Ig (D2HGDH Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66364-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat heart tissue using D2HGDH Monoclonal antibody (66364-1-Ig)

    WB analysis of rat heart using 66364-1-Ig (same clone as 66364-1-PBS)

    rat heart tissue were subjected to SDS PAGE followed by western blot with 66364-1-Ig (D2HGDH Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66364-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using D2HGDH Monoclonal antibody (66364-1-Ig)

    IHC staining of human liver cancer using 66364-1-Ig (same clone as 66364-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66364-1-Ig (D2HGDH Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66364-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using D2HGDH Monoclonal antibody (66364-1-Ig)

    IHC staining of human liver cancer using 66364-1-Ig (same clone as 66364-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66364-1-Ig (D2HGDH Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66364-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using D2HGDH Monoclonal antibody (66364-1-Ig)

    IF Staining of HepG2 using 66364-1-Ig (same clone as 66364-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using D2HGDH antibody (66364-1-Ig, Clone: 1E9B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66364-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using D2HGDH Monoclonal antibody (66364-1-Ig)

    IF Staining of HepG2 using 66364-1-Ig (same clone as 66364-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using D2HGDH antibody (66364-1-Ig, Clone: 1E9B3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66364-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using D2HGDH Monoclonal antibody (66364-1-Ig)

    FC experiment of HepG2 using 66364-1-Ig (same clone as 66364-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human D2HGDH (66364-1-Ig, Clone:1E9B3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66364-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66364-1-PBS targets D2HGDH in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with rat, mouse, human samples.

Tested Reactivity rat, mouse, human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen D2HGDH fusion protein Ag5036 相同性解析による交差性が予測される生物種
Full Name D-2-hydroxyglutarate dehydrogenase
Calculated molecular weight 521 aa, 56 kDa
Observed molecular weight 56 kDa
GenBank accession numberBC036604
Gene symbol D2HGDH
Gene ID (NCBI) 728294
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

D2HGDH(D-2-hydroxyglutarate dehydrogenase, mitochondrial) is also named as D2HGD and belongs to the FAD-binding oxidoreductase/transferase type 4 family.It catalyzes the oxidation of D-2-hydroxyglutarate to alpha-ketoglutarate.Defects in D2HGDH are the cause of D-2-hydroxyglutaric aciduria type 1 (D2HGA1).