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Species Reactivity

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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DAB1 Polyclonal antibody (31459-1-AP)

    WB analysis using 31459-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31459-1-AP (DAB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using DAB1 Polyclonal antibody (31459-1-AP)

    WB analysis using 31459-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31459-1-AP (DAB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DAB1 Polyclonal antibody (31459-1-AP)

    IF Staining of HepG2 using 31459-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DAB1 antibody (31459-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

DAB1 Polyclonal antibody

DAB1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 31459-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, pig

Applications

WB, IF/ICC, ELISA

Synonyms

Disabled homolog 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inpig cerebellum tissue, MCF-7 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31459-1-AP targets DAB1 in WB, IF/ICC, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DAB1 fusion protein Ag35376 相同性解析による交差性が予測される生物種
Full Name disabled homolog 1 (Drosophila)
Calculated molecular weight64 kDa
Observed molecular weight45 kDa, 80 kDa
GenBank accession numberNM_021080
Gene Symbol DAB1
Gene ID (NCBI) 1600
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDO75553
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Disabled-1 (Dab1) is an adaptor protein that is essential for neuronal migration and maturation in response to the extracellular protein Reelin. DAB1 protein docks to the intracellular part of the Reelin very low density lipoprotein receptor and apoE receptor type 2 and becomes tyrosine-phosphorylated following binding of Reelin to cortical neurons. The DAB1 protein contains a 180-amino acid N-terminal protein interaction/phosphotyrosine-binding (PTB) 1 domain that docks to the short cytoplasmic tail of the very low density lipoprotein receptor or apoE receptor type 2 at the level of NPXY motifs, with a preference for unphosphorylated motifs. Dab1 polypeptide bands ranging from 36 to 120 kDa have been identified in mouse embryonic brain, the 80-kDa Dab1 being the predominant form (PMID: 24313315).

Protocols

Product Specific Protocols
WB protocol for DAB1 antibody 31459-1-APDownload protocol
IF protocol for DAB1 antibody 31459-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols