• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using DAB2IP Polyclonal antibody (30020-1-AP)

    WB analysis using 30020-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30020-1-AP (DAB2IP antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using DAB2IP Polyclonal antibody (30020-1-AP)

    WB analysis using 30020-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30020-1-AP (DAB2IP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of 4T1 cells using DAB2IP Polyclonal antibody (30020-1-AP)

    IP experiment of 4T1 using 30020-1-AP

    IP result of anti-DAB2IP (IP:30020-1-AP, 4ug; Detection:30020-1-AP 1:6000) with 4T1 cells lysate 2800 ug.

  • Immunoprecipitation (IP) experiment of 4T1 cells using DAB2IP Polyclonal antibody (30020-1-AP)

    IP experiment of 4T1 using 30020-1-AP

    IP result of anti-DAB2IP (IP:30020-1-AP, 4ug; Detection:30020-1-AP 1:6000) with 4T1 cells lysate 4800 ug.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

DAB2IP Polyclonal antibody

DAB2IP Polyclonal Antibody for WB, IP, ELISA
Cat No. 30020-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

DAB2 interacting protein, ASK-interacting protein 1, ASK interacting protein 1, AIP-1, AIP1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, HepG2 cells, rat brain tissue
Positive IP detected in4T1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30020-1-AP targets DAB2IP in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DAB2IP fusion protein Ag31340 相同性解析による交差性が予測される生物種
Full Name DAB2 interacting protein
Calculated molecular weight 1189 aa, 132 kDa
Observed molecular weight 125-132 kDa
GenBank accession numberBC146762
Gene Symbol DAB2IP
Gene ID (NCBI) 153090
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5VWQ8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Disabled homolog 2-interacting protein (DAB2IP) is also named as ASK-interacting protein 1 (AIP-1). DAB2IP, is a new member of the Ras GTPase-activating protein family. It interacts directly with DAB2 and has distinct cellular functions such as modulating different signal cascades associated with cell proliferation, survival, apoptosis and metastasis. The aberrant alteration of DAB2IP in cancer is caused by a variety of mechanisms, including the aberrant promoter methylation, histone deacetylation, and others (PMID: 26658103). DAB2IP is reported to bind with Apoptosis signal-regulating kinase 1 (ASK1), and is called ASK1 Interacting Protein (AIP1) (PMID: 12813029). DAB2IP function is that of a tumor suppressor in tumor cell growth, metastasis, and other aspects in cancer progression. In prostate cancer, DAB2IP expression was repressed by promoter methylation and histone modification (PMID: 12446720, PMID: 15817459).

Protocols

Product Specific Protocols
WB protocol for DAB2IP antibody 30020-1-APDownload protocol
IP protocol for DAB2IP antibody 30020-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols