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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DACH2 Polyclonal antibody

DACH2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 25219-1-AP

Synonyms

DACH2, Dachshund homolog 2

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse heart tissue using DACH2 Polyclonal antibody (25219-1-AP)

    WB analysis of mouse heart using 25219-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 25219-1-AP (DACH2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using DACH2 Polyclonal antibody (25219-1-AP)

    WB analysis of NIH/3T3 using 25219-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25219-1-AP (DACH2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using DACH2 Polyclonal antibody (25219-1-AP)

    WB analysis of mouse kidney using 25219-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 25219-1-AP (DACH2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse eye tissue using DACH2 Polyclonal antibody (25219-1-AP)

    WB analysis of mouse eye using 25219-1-AP

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 25219-1-AP (DACH2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using DACH2 Polyclonal antibody (25219-1-AP)

    WB analysis of HeLa using 25219-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25219-1-AP (DACH2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inmouse heart tissue, HeLa cells, mouse eye tissue, mouse kidney tissue, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25219-1-AP is DACH2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DACH2 fusion protein Ag18403 相同性解析による交差性が予測される生物種
Full Name dachshund homolog 2 (Drosophila)
Calculated molecular weight 599 aa, 65 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC114950
Gene symbol DACH2
Gene ID (NCBI) 117154
RRIDAB_2879967
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Dachshund homolog 2 (DACH2), is a 599 amino acid protein, which belongs to the DACH family. DACH2 localizes in the nucleus and as a transcription factor is involved in regulation of organogenesis.

Protocols

Product Specific Protocols
WB protocol for DACH2 antibody 25219-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols