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Dystroglycan Polyclonal antibody

Dystroglycan Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 11017-1-AP

Synonyms

DAG1, DAG, Beta DG, Alpha DG Beta dystroglycan, AGRNR


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Tested Applications

Positive WB detected inHepG2 cells, mouse skeletal muscle tissue, mouse brain tissue, HeLa cells
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse heart tissue, mouse skeletal muscle tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11017-1-AP targets Dystroglycan in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Dystroglycan fusion protein Ag1456 相同性解析による交差性が予測される生物種
Full Name dystroglycan 1 (dystrophin-associated glycoprotein 1)
Calculated molecular weight 97 kDa
Observed molecular weight 40-43 kDa
GenBank accession numberBC012740
Gene symbol Dystroglycan
Gene ID (NCBI) 1605
RRIDAB_2090032
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Dystroglycan, also known as DAG1 or DG, was originally isolated from skeletal muscle as an integral membrane component of the dystrophin-glycoprotein complex (DGC). In addition to skeletal muscle, dystroglycan is strongly expressed in heart and smooth muscle, as well as many non-muscle tissues including brain and peripheral nerve (PMID: 12556455). The dystroglycan is involved in a number of processes including laminin and basement membrane assembly, sarcolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization. Dystroglycan consists of two subunits (alpha and beta), which are translated from a single mRNA as a propeptide that is proteolytically cleaved into two noncovalently associated proteins (PMID: 16410545). Alpha-dystroglycan is a 156-kDa extracellular peripheral glycoprotein, while beta-dystroglycan is a 43-kDa transmembrane protein (PMID: 9858474). The 43-kDa beta-dystroglycan can be cleaved into a ~30-kDa form (PMID: 14678802; 18458097; 17255331).

Protocols

Product Specific Protocols
WB protocol for Dystroglycan antibody 11017-1-APDownload protocol
IHC protocol for Dystroglycan antibody 11017-1-APDownload protocol
IF protocol for Dystroglycan antibody 11017-1-APDownload protocol
IP protocol for Dystroglycan antibody 11017-1-APDownload protocol
FC protocol for Dystroglycan antibody 11017-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Nat Commun

IL-20 antagonist suppresses PD-L1 expression and prolongs survival in pancreatic cancer models.

Authors - Shao-Wei Lu
mouseWB

Transl Res

AAV-based gene therapy prevents and halts the progression of dilated cardiomyopathy in a mouse model of phosphoglucomutase I deficiency (PGM1-CDG)

Authors - B Balakrishnan
humanWB

Mol Oncol

Circular RNA circ-TNRC6B inhibits the proliferation and invasion of esophageal squamous cell carcinoma cells by regulating the miR-452-5p/DAG1 axis

Authors - Ruiyu Xu
humanWB

Cell Commun Signal

Wnt-driven LARGE2 mediates laminin-adhesive O-glycosylation in human colonic epithelial cells and colorectal cancer.

Authors - Vanessa Dietinger
mouseIHC

Front Cardiovasc Med

Determination of Agrin and Related Proteins Levels as a Function of Age in Human Hearts.

Authors - Katie L Skeffington
humanIHC

J Med Virol

Identification of potential plasma markers for hepatitis B virus-related chronic hepatitis and liver fibrosis/cirrhosis.

Authors - Dandan Zhao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Perrin (Verified Customer) (11-22-2022)

Works very well, with little background

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1 to 100
  • Cell Tissue Type: Gut slice, whole mount
FH

Ryan (Verified Customer) (01-11-2018)

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Zebrafish Retina
Dystroglycan Antibody Immunohistochemistry, validation (1:100 dilution) in Zebrafish Retina (Cat no:11017-1-AP)