Validation Data Gallery

Filter: AllWBIPIHC

WB analysis of HeLa using 12224-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12224-1-AP (DAK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 12224-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 12224-1-AP (DAK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 12224-1-AP
WB result of DAK antibody (12224-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-DAK transfected HeLa cells.
WB analysis using 12224-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12224-1-AP (DAK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of mouse uterus using 12224-1-AP
mouse uterus tissue were subjected to SDS PAGE followed by western blot with 12224-1-AP (DAK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 12224-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12224-1-AP (DAK Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 12224-1-AP
IP result of anti-DAK (IP:12224-1-AP, 3ug; Detection:12224-1-AP 1:500) with HeLa cells lysate 2150ug.
IHC staining of human stomach using 12224-1-AP
Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 12224-1-AP (DAK antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

DAK Polyclonal antibody

DAK Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 12224-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

FAD-AMP lyase, DHA kinase, cyclizing, cyclic FMN forming, Bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (4)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, HEK-293 cells, mouse uterus tissue, HepG2 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human stomach tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 4 publications below
IF	See 1 publications below

Product Information

12224-1-AP targets DAK in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	DAK fusion protein Ag2861 相同性解析による交差性が予測される生物種
Full Name	dihydroxyacetone kinase 2 homolog (S. cerevisiae)
Calculated molecular weight	575 aa, 59 kDa
Observed molecular weight	55-59 kDa
GenBank accession number	BC001341
Gene Symbol	DAK
Gene ID (NCBI)	26007
RRID	AB_2277108
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q3LXA3
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

DAK, also name as TKFC, Triokinase, and FMN cyclase, catalyzes both the phosphorylation of dihydroxyacetone and of glyceraldehyde, and the splitting of ribonucleoside diphosphate-X compounds among which FAD is the best substrate. DAK has some isoforms with MW 51-55 kDa and 59 kDa.

Protocols

Product Specific Protocols
WB protocol for DAK antibody 12224-1-AP	Download protocol
IHC protocol for DAK antibody 12224-1-AP	Download protocol
IP protocol for DAK antibody 12224-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Endocrinology Hepatic but not intestinal FBP1 is required for fructose metabolism and tolerance Authors - Qiufang Bai View Article
human	WB,IF	iScience CircSOBP suppresses the progression of glioma by disrupting glycolysis and promoting the MDA5-mediated immune response Authors - Maolin Mu KO Validated View Article
rat	WB	Mol Psychiatry Proteasome-independent K63 polyubiquitination selectively regulates ATP levels and proteasome activity during fear memory formation in the female amygdala Authors - Kayla Farrell View Article
human	WB	Pharmaceuticals (Basel) Magnolol Inhibits High Fructose-Induced Podocyte Inflammation via Downregulation of TKFC/Sp1/HDAC4/Notch1 Activation Authors - Ziang Zhou View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 12224-1-AP

WB analysis of HEK-293 using 12224-1-AP

WB analysis of HeLa using 12224-1-AP

WB analysis using 12224-1-AP

WB analysis of mouse uterus using 12224-1-AP

WB analysis of HeLa using 12224-1-AP

IP experiment of HeLa using 12224-1-AP

IHC staining of human stomach using 12224-1-AP

DAK Polyclonal antibody

DAK Polyclonal Antibody for WB, IHC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Hepatic but not intestinal FBP1 is required for fructose metabolism and tolerance

CircSOBP suppresses the progression of glioma by disrupting glycolysis and promoting the MDA5-mediated immune response

Proteasome-independent K63 polyubiquitination selectively regulates ATP levels and proteasome activity during fear memory formation in the female amygdala

Magnolol Inhibits High Fructose-Induced Podocyte Inflammation via Downregulation of TKFC/Sp1/HDAC4/Notch1 Activation