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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DAO Monoclonal antibody

DAO Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1C12F4

Cat no : 66296-1-Ig

Synonyms

D amino acid oxidase, DAAO, DAMOX, DAO, OXDA

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DAO Monoclonal antibody (66296-1-Ig)

    WB analysis using 66296-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66296-1-Ig (DAO antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of fetal human brain tissue using DAO Monoclonal antibody (66296-1-Ig)

    WB analysis of fetal human brain using 66296-1-Ig

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66296-1-Ig (DAO Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using DAO Monoclonal antibody (66296-1-Ig)

    IHC staining of human gliomas using 66296-1-Ig

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66296-1-Ig (DAO Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using DAO Monoclonal antibody (66296-1-Ig)

    IHC staining of human gliomas using 66296-1-Ig

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66296-1-Ig (DAO Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using DAO Monoclonal antibody (66296-1-Ig)

    IHC staining of mouse cerebellum using 66296-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66296-1-Ig (DAO antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using DAO Monoclonal antibody (66296-1-Ig)

    IHC staining of mouse cerebellum using 66296-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66296-1-Ig (DAO antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inpig brain tissue, fetal human brain tissue, pig cerebellum tissue, rat brain tissue, rat cerebellum tissue
Positive IHC detected inhuman gliomas tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66296-1-Ig targets DAO in WB, IHC, ELISA applications and shows reactivity with human, pig, rat samples.

Tested Reactivity human, pig, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DAO fusion protein Ag6928 相同性解析による交差性が予測される生物種
Full Name D-amino-acid oxidase
Calculated molecular weight 347 aa, 39 kDa
Observed molecular weight 39 kDa
GenBank accession numberBC029057
Gene symbol DAO
Gene ID (NCBI) 1610
RRIDAB_2881679
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

D-amino acid oxidase (DAO),with MW of 39 kDa, is a flavoprotein which uses flavin adenine dinucleotide as its prosthetic group. Its substrates include a wide variety of D-amino acids, but it is inactive on the naturally occurring L-amino acids. DAO may play a role in the pathophysiology of schizophrenia and act as a detoxifying agent which removes D-amino acids that accumulate during aging.DAAO exists in solution as a mixture of monomer-dimer(80 kDa) and even higher molecular aggregates in equilibrium(170 kDa), depending on the enzyme concentration and forms(PMID: 11130179/PMID: 9210639).

Protocols

Product Specific Protocols
WB protocol for DAO antibody 66296-1-IgDownload protocol
IHC protocol for DAO antibody 66296-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols