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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DAP3 Polyclonal antibody

DAP3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10276-1-AP

Synonyms

MRPS29, MRP S29, Death-associated protein 3, DAP-3, DAP 3

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using DAP3 Polyclonal antibody (10276-1-AP)

    WB analysis of HeLa using 10276-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10276-1-AP (DAP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using DAP3 Polyclonal antibody (10276-1-AP)

    WB analysis of HeLa using 10276-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10276-1-AP (DAP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using DAP3 Polyclonal antibody (10276-1-AP)

    WB analysis of human brain using 10276-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 10276-1-AP (DAP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using DAP3 Polyclonal antibody (10276-1-AP)

    IHC staining of human colon using 10276-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10276-1-AP (DAP3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach tissue using DAP3 Polyclonal antibody (10276-1-AP)

    IHC staining of human stomach using 10276-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10276-1-AP (DAP3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHeLa cells, human brain tissue
Positive IHC detected inhuman stomach tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

10276-1-AP targets DAP3 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DAP3 fusion protein Ag0274 相同性解析による交差性が予測される生物種
Full Name death associated protein 3
Calculated molecular weight 46 kDa
Observed molecular weight 40-45 kDa
GenBank accession numberBF220193
Gene symbol DAP3
Gene ID (NCBI) 7818
RRIDAB_10695891
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DAP3 antibody 10276-1-APDownload protocol
IHC protocol for DAP3 antibody 10276-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Noncoding RNA Res

DNA methylation of microRNA-365-1 induces apoptosis of hair follicle stem cells by targeting DAP3

Authors - Xin Liu
View Article