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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using DAP3 Polyclonal antibody (10276-1-AP)

    WB analysis of HeLa using 10276-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10276-1-AP (DAP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using DAP3 Polyclonal antibody (10276-1-AP)

    WB analysis of HeLa using 10276-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10276-1-AP (DAP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using DAP3 Polyclonal antibody (10276-1-AP)

    WB analysis of human brain using 10276-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 10276-1-AP (DAP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using DAP3 Polyclonal antibody (10276-1-AP)

    IHC staining of human colon using 10276-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10276-1-AP (DAP3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach tissue using DAP3 Polyclonal antibody (10276-1-AP)

    IHC staining of human stomach using 10276-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10276-1-AP (DAP3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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DAP3 Polyclonal antibody

DAP3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 10276-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

MRPS29, MRP S29, Death-associated protein 3, DAP-3, DAP 3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, human brain tissue
Positive IHC detected inhuman stomach tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10276-1-AP targets DAP3 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DAP3 fusion protein Ag0274 相同性解析による交差性が予測される生物種
Full Name death associated protein 3
Calculated molecular weight 46 kDa
Observed molecular weight 40-45 kDa
GenBank accession numberBF220193
Gene Symbol DAP3
Gene ID (NCBI) 7818
RRIDAB_10695891
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP51398
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DAP3 antibody 10276-1-APDownload protocol
IHC protocol for DAP3 antibody 10276-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

iScience

Supernumerary proteins of the human mitochondrial ribosomal small subunit are integral for assembly and translation

Authors - Taru Hilander
View Article
mouseWB

Noncoding RNA Res

DNA methylation of microRNA-365-1 induces apoptosis of hair follicle stem cells by targeting DAP3

Authors - Xin Liu
View Article