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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using DAPK1 Polyclonal antibody (19938-1-AP)

    WB analysis of HepG2 using 19938-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 19938-1-AP (DAPK1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using DAPK1 Polyclonal antibody (19938-1-AP)

    IHC staining of human small intestine using 19938-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 19938-1-AP (DAPK1 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using DAPK1 Polyclonal antibody (19938-1-AP)

    IHC staining of human small intestine using 19938-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 19938-1-AP (DAPK1 Antibody) at dilution of 1:200 (under 40x lens).

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DAPK1 Polyclonal antibody

DAPK1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 19938-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Synonyms

DAP kinase 1, DAPK, DAPK1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

19938-1-AP targets DAPK1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name death-associated protein kinase 1
Calculated molecular weight 160 kDa
GenBank accession numberNM_004938
Gene Symbol DAPK1
Gene ID (NCBI) 1612
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP53355
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DAPK1, also named DAPK, belongs to the protein kinase superfamily; CAMK Ser/Thr protein kinase family and DAP kinase subfamily. DAPK1 is a calcium/calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis. It catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein. The antibody recognizes isoform1 of DAPK1.

Protocols

Product Specific Protocols
WB protocol for DAPK1 antibody 19938-1-APDownload protocol
IHC protocol for DAPK1 antibody 19938-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

J Tradit Complement Med

Effect of electrode configuration in electroacupuncture on ischemic stroke treatment in rats

Authors - Chung-Hsiang Liu
View Article