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  • Featured Product
  • KD/KO Validated

DAPK2-Specific Polyclonal antibody

DAPK2-Specific Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20048-1-AP

Synonyms

DAPK2, DRP 1, Death-associated protein kinase 2, DAP-kinase-related protein 1, DAP kinase related protein 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DAPK2-Specific Polyclonal antibody (20048-1-AP)

    WB analysis using 20048-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20048-1-AP (DAPK2-Specific antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using DAPK2-Specific Polyclonal antibody (20048-1-AP)

    WB analysis of HeLa using 20048-1-AP

    WB result of DAPK2-Specific antibody (20048-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DAPK2-Specific transfected HeLa cells.

  • Western Blot (WB) analysis of A431 cells using DAPK2-Specific Polyclonal antibody (20048-1-AP)

    WB analysis of A431 using 20048-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 20048-1-AP (DAPK2-Specific antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using DAPK2-Specific Polyclonal antibody (20048-1-AP)

    WB analysis of A431 using 20048-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 20048-1-AP (DAPK2-Specific antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A375 cells using DAPK2-Specific Polyclonal antibody (20048-1-AP)

    IF Staining of A375 using 20048-1-AP

    Immunofluorescent analysis of A375 cells using 20048-1-AP (DAPK2-Specific antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells, A431 cells, HepG2 cells
Positive IF/ICC detected inA375 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20048-1-AP targets DAPK2-Specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name death-associated protein kinase 2
Calculated molecular weight 43 kDa
Observed molecular weight 38-43 kDa
GenBank accession numberNM_014326
Gene symbol DAPK2
Gene ID (NCBI) 23604
RRIDAB_10667409
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DAPK2, also named as DRP-1, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and DAP kinase subfamily. DAPK2 is a calcium/calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis. DAPK2 catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein. The antibody is specific to DAPK2.

Protocols

Product Specific Protocols
WB protocol for DAPK2-Specific antibody 20048-1-APDownload protocol
IF protocol for DAPK2-Specific antibody 20048-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

J Cancer

Identification and validation of a novel apoptosis-related prognostic risk score model for lung adenocarcinoma

Authors - Yan Wang
View Article