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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DARS Monoclonal antibody

DARS Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3C5D3

Cat no : 67930-1-Ig

Synonyms

Aspartate tRNA ligase, aspartyl tRNA synthetase, AspRS, DARS, DKFZp781B11202

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DARS Monoclonal antibody (67930-1-Ig)

    WB analysis using 67930-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67930-1-Ig (DARS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat cerebellum tissue using DARS Monoclonal antibody (67930-1-Ig)

    IHC staining of rat cerebellum using 67930-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 67930-1-Ig (DARS antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using DARS Monoclonal antibody (67930-1-Ig)

    IF Staining of SH-SY5Y using 67930-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using DARS antibody (67930-1-Ig, Clone: 3C5D3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells
Positive IHC detected inrat cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67930-1-Ig targets DARS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen DARS fusion protein Ag7059 相同性解析による交差性が予測される生物種
Full Name aspartyl-tRNA synthetase
Calculated molecular weight 57 kDa
Observed molecular weight50-57 kDa
GenBank accession numberBC000629
Gene symbol DARS
Gene ID (NCBI) 1615
RRIDAB_2918682
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DARS antibody 67930-1-IgDownload protocol
IHC protocol for DARS antibody 67930-1-IgDownload protocol
IF protocol for DARS antibody 67930-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols