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- KD/KO Validated
DARS2 Polyclonal antibody
DARS2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 13807-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | K-562 cells, human placenta tissue, U-937 cells |
| Positive IHC detected in | mouse cerebellum tissue, human brain tissue, human testis tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 8 publications below |
| IHC | See 3 publications below |
Product Information
13807-1-AP targets DARS2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | DARS2 fusion protein Ag4809 相同性解析による交差性が予測される生物種 |
| Full Name | aspartyl-tRNA synthetase 2, mitochondrial |
| Calculated molecular weight | 645 aa, 74 kDa |
| Observed molecular weight | 66-74 kDa |
| GenBank accession number | BC045173 |
| Gene symbol | DARS2 |
| Gene ID (NCBI) | 55157 |
| RRID | AB_2088759 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DARS2 is also named as AspRS (aspartyl-tRNA synthetase) and belongs to the class-II aminoacyl-tRNA synthetase family. The deduced 645-amino acid protein has a 47-amino acid mitochondrial targeting signal, resulting in a mature protein of 598 amino acids. DARS2 contains conserved residues involved in ATP binding, tRNA binding, and aspartic acid recognition, as well as catalytic site motifs characteristic of amino acid tRNA synthetases. It is a dimeric proteins(PMID:19443655). Rat aspartyl-tRNA synthetase has a N-terminal polypeptide extension of about 40 amino acid residues which can be removed without impairing its catalytic activity(PMID:9030747). This protein has different molecular mass(55 kDa, 50 kDa,66 kDa ) according to the publications(PMID:11306575; 19443655;15299749).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DARS2 antibody 13807-1-AP | Download protocol |
| IHC protocol for DARS2 antibody 13807-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Metab Mitochondrial metabolism coordinates stage-specific repair processes in macrophages during wound healing. | ||
Cell Metab Tissue-Specific Loss of DARS2 Activates Stress Responses Independently of Respiratory Chain Deficiency in the Heart. | ||
EMBO Rep Loss of CLPP alleviates mitochondrial cardiomyopathy without affecting the mammalian UPRmt.
| ||
Am J Cancer Res DARS2 is a prognostic biomarker and correlated with immune infiltrates and cuproptosis in lung adenocarcinoma
| ||
Int J Biochem Cell Biol Mitochondrial oxidative phosphorylation is impaired in TALLYHO mice, a new obesity and type 2 diabetes animal model. | ||
Thorac Cancer DARS2 promotes the occurrence of lung adenocarcinoma via the ERK/c-Myc signaling pathway
|
