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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DAX-1 Polyclonal antibody, PBS Only

DAX-1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 20849-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using DAX-1 Polyclonal antibody (20849-1-AP)

    WB analysis of MCF-7 using 20849-1-AP (same clone as 20849-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 20849-1-AP (DAX-1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20849-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using DAX-1 Polyclonal antibody (20849-1-AP)

    IHC staining of human small intestine using 20849-1-AP (same clone as 20849-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20849-1-AP (DAX-1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20849-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using DAX-1 Polyclonal antibody (20849-1-AP)

    IHC staining of human testis using 20849-1-AP (same clone as 20849-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20849-1-AP (DAX-1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20849-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using DAX-1 Polyclonal antibody (20849-1-AP)

    IHC staining of human small intestine using 20849-1-AP (same clone as 20849-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20849-1-AP (DAX-1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20849-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using DAX-1 Polyclonal antibody (20849-1-AP)

    IHC staining of human testis using 20849-1-AP (same clone as 20849-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20849-1-AP (DAX-1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20849-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using DAX-1 Polyclonal antibody (20849-1-AP)

    IF Staining of MCF-7 using 20849-1-AP (same clone as 20849-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed MCF-7 cells using 20849-1-AP (DAX-1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20849-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20849-1-PBS targets DAX-1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DAX-1 fusion protein Ag14416 相同性解析による交差性が予測される生物種
Full Name nuclear receptor subfamily 0, group B, member 1
Calculated molecular weight 470 aa, 52 kDa
Observed molecular weight 54 kDa
GenBank accession numberBC011564
Gene symbol NR0B1
Gene ID (NCBI) 190
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.