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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DAXX Polyclonal antibody, PBS Only

DAXX Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 20489-1-PBS

Synonyms

ETS1 associated protein 1, EAP1, Death domain-associated protein 6, Death domain associated protein 6, DAP6

Validation Data Gallery

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  • Western Blot (WB) analysis of Y79 cells using DAXX Polyclonal antibody (20489-1-AP)

    WB analysis of Y79 using 20489-1-AP (same clone as 20489-1-PBS)

    Y79 cells were subjected to SDS PAGE followed by western blot with 20489-1-AP (DAXX antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20489-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using DAXX Polyclonal antibody (20489-1-AP)

    WB analysis of HeLa using 20489-1-AP (same clone as 20489-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 20489-1-AP (DAXX antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20489-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using DAXX Polyclonal antibody (20489-1-AP)

    IHC staining of human stomach using 20489-1-AP (same clone as 20489-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 20489-1-AP (DAXX antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20489-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20489-1-PBS targets DAXX in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DAXX fusion protein Ag14519 相同性解析による交差性が予測される生物種
Full Name death-domain associated protein
Calculated molecular weight 740 aa, 81 kDa
Observed molecular weight 120 kDa, 70 kDa
GenBank accession numberBC109074
Gene symbol DAXX
Gene ID (NCBI) 1616
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Death associated protein 6 (DAXX) is a ubiquitous protein implicated in various cellular processes such as apoptosis, tumorigenesis, development and transcription. It has been shown to translocate from the nucleus to the cytoplasm under conditions of stress and to activate the Jun N-terminal kinase (JNK) pathway. Somatic mutations of death associated protein (DAXX) have been found in ALT cancers, including pancreatic neuroendocrine (panNET) cancers and glioblastoma multiforme (GBM) cancers.