DAXX Monoclonal antibody
DAXX Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Human
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
1F3G9
Cat no : 67879-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Raji cells, HeLa cells, Daudi cells, MOLT-4 cells, Jurkat cells |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | A431 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
67879-1-Ig targets DAXX in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | DAXX fusion protein Ag14606 相同性解析による交差性が予測される生物種 |
Full Name | death-domain associated protein |
Calculated molecular weight | 740 aa, 81 kDa |
Observed molecular weight | 100-130 kDa |
GenBank accession number | BC109074 |
Gene symbol | DAXX |
Gene ID (NCBI) | 1616 |
RRID | AB_2918636 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
Death domain-associated protein (DAXX) is a transcription repressor involved in both physiological and pathological conditions. DAXX overexpression is a common feature in diverse cancers, which correlates with tumorigenesis, disease progression and treatment resistance. Daxx migrated with an apparent molecular weight of approximately 100-130 kDa. (PMID: 31350900, PMID: 32203224, PMID: 31614769)
Protocols
Product Specific Protocols | |
---|---|
WB protocol for DAXX antibody 67879-1-Ig | Download protocol |
IHC protocol for DAXX antibody 67879-1-Ig | Download protocol |
IF protocol for DAXX antibody 67879-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |