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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using DAZ4 Polyclonal antibody (14065-1-AP)

    WB analysis of mouse testis using 14065-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 14065-1-AP (DAZ4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat testis tissue using DAZ4 Polyclonal antibody (14065-1-AP)

    WB analysis of rat testis using 14065-1-AP

    rat testis tissue were subjected to SDS PAGE followed by western blot with 14065-1-AP (DAZ4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using DAZ4 Polyclonal antibody (14065-1-AP)

    WB analysis of mouse testis using 14065-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 14065-1-AP (DAZ4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using DAZ4 Polyclonal antibody (14065-1-AP)

    WB analysis of human testis using 14065-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 14065-1-AP (DAZ4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using DAZ4 Polyclonal antibody (14065-1-AP)

    IHC staining of human stomach using 14065-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 14065-1-AP (DAZ4 antibody) at dilution of 1:120 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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DAZ4 Polyclonal antibody

DAZ4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14065-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

pDP1681, pDP1680, DAZ1, DAZ 4, DAZ

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse testis tissue, human testis tissue, rat testis tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14065-1-AP targets DAZ4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DAZ4 fusion protein Ag5116 相同性解析による交差性が予測される生物種
Full Name deleted in azoospermia 4
Calculated molecular weight 65 kDa
Observed molecular weight 60-65 kDa
GenBank accession numberBC047617
Gene Symbol DAZ4
Gene ID (NCBI) 57135
RRIDAB_2878008
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86SG3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DAZ4, encoded by the DAZ gene, is an RNA-binding protein with a role in spermatogenesis. DAZ4 is a testis-specific protein that expression restricted to premeiotic germ cells, particularly in spermatogonia. Defects in DAZ4 may be a cause of spermatogenic failure Y-linked type 2 (SPGFY2). It is a disorder resulting in the absence (azoospermia) or reduction (oligozoospermia) of sperm in the semen, leading to male infertility.

Protocols

Product Specific Protocols
WB protocol for DAZ4 antibody 14065-1-APDownload protocol
IHC protocol for DAZ4 antibody 14065-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols