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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DAZAP2 Polyclonal antibody (15210-1-AP)

    WB analysis using 15210-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15210-1-AP (DAZAP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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DAZAP2 Polyclonal antibody

DAZAP2 Polyclonal Antibody for WB, ELISA
Cat No. 15210-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

PRTB, Proline-rich transcript in brain protein, KIAA0058, Deleted in azoospermia-associated protein 2, DAZ-associated protein 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA375 cells, C6 cells, J774A.1 cells, RAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15210-1-AP targets DAZAP2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DAZAP2 fusion protein Ag7364 相同性解析による交差性が予測される生物種
Full Name DAZ associated protein 2
Calculated molecular weight 17 kDa
Observed molecular weight 17 kDa, 22 kDa
GenBank accession numberBC002334
Gene Symbol DAZAP2
Gene ID (NCBI) 9802
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15038
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DAZAP2, also named KIAA0058, has six isoforms. In unstressed cells, it promotes SIAH1-mediated polyubiquitination and degradation of the serine/threonine-protein kinase HIPK2, probably by acting as a loading factor that potentiates complex formation between HIPK2 and ubiquitin ligase SIAH1. In response to DNA damage, it localizes to the nucleus following phosphorylation by HIPK2 and modulates the expression of a subset of TP53/p53 target genes by binding to TP53 at target gene promoters (PMID: 33591310).

Protocols

Product Specific Protocols
WB protocol for DAZAP2 antibody 15210-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols