DAZL Polyclonal antibody

Name: DAZL Polyclonal antibody
Brand: Proteintech
SKU: 12633-1-AP

DAZL Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 12633-1-AP

Synonyms

Deleted in azoospermia-like 1, Deleted in azoospermia-like, DAZ-like autosomal, DAZLA, DAZL1

Validation Data Gallery

Filter:

AllWBIPIHCIF-P

WB analysis using 12633-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12633-1-AP (DAZL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IP experiment of mouse testis using 12633-1-AP
IP result of anti-DAZL (IP:12633-1-AP, 4ug; Detection:12633-1-AP 1:500) with mouse testis tissue lysate 4400ug.
IHC staining of mouse testis using 12633-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12633-1-AP (DAZL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse ovary using 12633-1-AP
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 12633-1-AP (DAZL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse ovary using 12633-1-AP
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 12633-1-AP (DAZL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 12633-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12633-1-AP (DAZL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse testis using 12633-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using DAZL antibody (12633-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse testis using 12633-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using DAZL antibody (12633-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse testis using 12633-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using DAZL antibody (12633-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 12633-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	rat testis tissue
Positive IP detected in	mouse testis tissue
Positive IHC detected in	mouse testis tissue, mouse ovary tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse testis tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below
IF	See 2 publications below

Product Information

12633-1-AP targets DAZL in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	DAZL fusion protein Ag3326 相同性解析による交差性が予測される生物種
Full Name	deleted in azoospermia-like
Calculated molecular weight	295 aa, 33 kDa
Observed molecular weight	33 kDa
GenBank accession number	BC027595
Gene symbol	DAZL
Gene ID (NCBI)	1618
RRID	AB_2277011
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for DAZL antibody 12633-1-AP	Download protocol
IHC protocol for DAZL antibody 12633-1-AP	Download protocol
IF protocol for DAZL antibody 12633-1-AP	Download protocol
IP protocol for DAZL antibody 12633-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB,IF	Environ Pollut Wnt10a downregulation contributes to MEHP-induced disruption of self-renewal and differentiation balance and proliferation inhibition in GC-1 cells: Insights from multiple transcriptomic profiling Authors - Yifan Hong View Article
rat	IF	Stem Cell Res Ther Concentrated exosomes from menstrual blood-derived stromal cells improves ovarian activity in a rat model of premature ovarian insufficiency. Authors - Siwen Zhang View Article
human,mouse	WB	Proteomics Systematic in-depth proteomic analysis of mitochondria-associated endoplasmic reticulum membranes (MAM) in mouse and human testes. Authors - Xiaoli Wang View Article
mouse	WB	Front Endocrinol (Lausanne) Restoring Impaired Fertility Through Diet: Observations of Switching From High-Fat Diet During Puberty to Normal Diet in Adulthood Among Obese Male Mice. Authors - Xiangyu Qi View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DAZL Polyclonal antibody

DAZL Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Validation Data Gallery

WB analysis using 12633-1-AP

IP experiment of mouse testis using 12633-1-AP

IHC staining of mouse testis using 12633-1-AP

IHC staining of mouse ovary using 12633-1-AP

IHC staining of mouse ovary using 12633-1-AP

IHC staining of mouse testis using 12633-1-AP

IF Staining of mouse testis using 12633-1-AP

IF Staining of mouse testis using 12633-1-AP

IF Staining of mouse testis using 12633-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Wnt10a downregulation contributes to MEHP-induced disruption of self-renewal and differentiation balance and proliferation inhibition in GC-1 cells: Insights from multiple transcriptomic profiling

Concentrated exosomes from menstrual blood-derived stromal cells improves ovarian activity in a rat model of premature ovarian insufficiency.

Systematic in-depth proteomic analysis of mitochondria-associated endoplasmic reticulum membranes (MAM) in mouse and human testes.

Restoring Impaired Fertility Through Diet: Observations of Switching From High-Fat Diet During Puberty to Normal Diet in Adulthood Among Obese Male Mice.